Abstract
Purpose: No information is currently available on genetic determinants of short-term response to drug withdrawal in medication overuse headache (MOH). In the present study, we aimed to evaluate the role of 14 polymorphisms in 8 candidate genes potentially relevant for drug addiction (OPRM1, DRD2, DBH, COMT, BDNF, SLC6A4, 5HT2A, and SLC1A2) as predictors for detoxification outcome of MOH patients at 2 months of follow-up. Methods: Genotyping was conducted by PCR, PCR-RFLP analysis, or real-time PCR allelic discrimination assay on genomic DNA extracted from peripheral blood. The association between gene variants and risk of unsuccessful detoxification was evaluated by univariate and multivariate logistic regression analyses. Results: One hundred and eight MOH patients with effective drug withdrawal therapy and 65 MOH patients with unsuccessful detoxification were available for the analysis. In the multivariable logistic regression analysis, triptan overuse (odds ratio (OR) 0.271, 95 % confidence interval (CI) 0.083-0.890, P∈=∈0.031) and TT genotype carriage of DRD2 NcoI (OR 0.115, 95 % CI 0.014-0.982, P∈=∈0.048) emerged as independent predictors for unsuccessful detoxification. In addition, carriers of at least four of the six top-ranked gene variants (P∈
| Original language | English |
|---|---|
| Pages (from-to) | 1195-1202 |
| Number of pages | 8 |
| Journal | European Journal of Clinical Pharmacology |
| Volume | 70 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Detoxification
- Drug withdrawal therapy
- Gene polymorphisms
- Medication overuse headache
- Outcome
- Risk
ASJC Scopus subject areas
- Pharmacology (medical)
- Pharmacology
- Medicine(all)
Cite this
Combined effect of common gene variants on response to drug withdrawal therapy in medication overuse headache. / Cargnin, Sarah; Viana, Michele; Sances, Grazia; Bianchi, Marika; Ghiotto, Natascia; Tassorelli, Cristina; Nappi, Giuseppe; Canonico, Pier Luigi; Genazzani, Armando A.; Terrazzino, Salvatore.
In: European Journal of Clinical Pharmacology, Vol. 70, No. 10, 2014, p. 1195-1202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Combined effect of common gene variants on response to drug withdrawal therapy in medication overuse headache
AU - Cargnin, Sarah
AU - Viana, Michele
AU - Sances, Grazia
AU - Bianchi, Marika
AU - Ghiotto, Natascia
AU - Tassorelli, Cristina
AU - Nappi, Giuseppe
AU - Canonico, Pier Luigi
AU - Genazzani, Armando A.
AU - Terrazzino, Salvatore
PY - 2014
Y1 - 2014
N2 - Purpose: No information is currently available on genetic determinants of short-term response to drug withdrawal in medication overuse headache (MOH). In the present study, we aimed to evaluate the role of 14 polymorphisms in 8 candidate genes potentially relevant for drug addiction (OPRM1, DRD2, DBH, COMT, BDNF, SLC6A4, 5HT2A, and SLC1A2) as predictors for detoxification outcome of MOH patients at 2 months of follow-up. Methods: Genotyping was conducted by PCR, PCR-RFLP analysis, or real-time PCR allelic discrimination assay on genomic DNA extracted from peripheral blood. The association between gene variants and risk of unsuccessful detoxification was evaluated by univariate and multivariate logistic regression analyses. Results: One hundred and eight MOH patients with effective drug withdrawal therapy and 65 MOH patients with unsuccessful detoxification were available for the analysis. In the multivariable logistic regression analysis, triptan overuse (odds ratio (OR) 0.271, 95 % confidence interval (CI) 0.083-0.890, P∈=∈0.031) and TT genotype carriage of DRD2 NcoI (OR 0.115, 95 % CI 0.014-0.982, P∈=∈0.048) emerged as independent predictors for unsuccessful detoxification. In addition, carriers of at least four of the six top-ranked gene variants (P∈
AB - Purpose: No information is currently available on genetic determinants of short-term response to drug withdrawal in medication overuse headache (MOH). In the present study, we aimed to evaluate the role of 14 polymorphisms in 8 candidate genes potentially relevant for drug addiction (OPRM1, DRD2, DBH, COMT, BDNF, SLC6A4, 5HT2A, and SLC1A2) as predictors for detoxification outcome of MOH patients at 2 months of follow-up. Methods: Genotyping was conducted by PCR, PCR-RFLP analysis, or real-time PCR allelic discrimination assay on genomic DNA extracted from peripheral blood. The association between gene variants and risk of unsuccessful detoxification was evaluated by univariate and multivariate logistic regression analyses. Results: One hundred and eight MOH patients with effective drug withdrawal therapy and 65 MOH patients with unsuccessful detoxification were available for the analysis. In the multivariable logistic regression analysis, triptan overuse (odds ratio (OR) 0.271, 95 % confidence interval (CI) 0.083-0.890, P∈=∈0.031) and TT genotype carriage of DRD2 NcoI (OR 0.115, 95 % CI 0.014-0.982, P∈=∈0.048) emerged as independent predictors for unsuccessful detoxification. In addition, carriers of at least four of the six top-ranked gene variants (P∈
KW - Detoxification
KW - Drug withdrawal therapy
KW - Gene polymorphisms
KW - Medication overuse headache
KW - Outcome
KW - Risk
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U2 - 10.1007/s00228-014-1726-6
DO - 10.1007/s00228-014-1726-6
M3 - Article
VL - 70
SP - 1195
EP - 1202
JO - European Journal of Clinical Pharmacology
JF - European Journal of Clinical Pharmacology
SN - 0031-6970
IS - 10
ER -