Combined HLA matched limbal stem cells allograft with amniotic membrane transplantation as a prophylactic surgical procedure to prevent corneal graft rejection after penetrating keratoplasty: Case report

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose. To determine if the use of combined HLA matched limbal stem cells allograft with amniotic membrane transplantation (AMT) is a safe and effective prophylactic surgical procedure to prevent corneal graft after penetrating keratoplasty (PK).

Methods. We report the case of a 17 years old patient with a history of congenital glaucoma, trabeculectomy and multiple corneal graft rejections, presenting total limbal cell deficiency. To reduce the possibility of graft rejection in the left eye after a new PK, a two step procedure was performed. At first the patient underwent a combined HLA matched limbal stem cells allograft (LAT) and AMT and then, 10 months later, a new PK.

Results. During 12 months of follow-up, the corneal graft remained stable and smooth, with no sign of graft rejection.

Conclusions. In our patient, the prophylactic use of LAT from HLA-matched donors and AMT before PK, may result in a better prognosis of corneal graft survival.

Original languageEnglish
Pages (from-to)298-300
Number of pages3
JournalAnnali dell'Istituto Superiore di Sanita
Volume50
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Penetrating Keratoplasty
Amnion
Graft Rejection
Allografts
Stem Cells
Transplantation
Transplants
Trabeculectomy
Graft Survival
Glaucoma
Tissue Donors
Prophylactic Surgical Procedures

Keywords

  • Amniotic membrane transplantation
  • Corneal transplantation
  • HLA matched allograft
  • Limbal stem cells

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

@article{41cabe124a354ffca4c165053b05df16,
title = "Combined HLA matched limbal stem cells allograft with amniotic membrane transplantation as a prophylactic surgical procedure to prevent corneal graft rejection after penetrating keratoplasty: Case report",
abstract = "Purpose. To determine if the use of combined HLA matched limbal stem cells allograft with amniotic membrane transplantation (AMT) is a safe and effective prophylactic surgical procedure to prevent corneal graft after penetrating keratoplasty (PK).Methods. We report the case of a 17 years old patient with a history of congenital glaucoma, trabeculectomy and multiple corneal graft rejections, presenting total limbal cell deficiency. To reduce the possibility of graft rejection in the left eye after a new PK, a two step procedure was performed. At first the patient underwent a combined HLA matched limbal stem cells allograft (LAT) and AMT and then, 10 months later, a new PK.Results. During 12 months of follow-up, the corneal graft remained stable and smooth, with no sign of graft rejection.Conclusions. In our patient, the prophylactic use of LAT from HLA-matched donors and AMT before PK, may result in a better prognosis of corneal graft survival.",
keywords = "Amniotic membrane transplantation, Corneal transplantation, HLA matched allograft, Limbal stem cells",
author = "Paolo Capozzi and Sergio Petroni and Luca Buzzonetti",
year = "2014",
doi = "10.4415/ANN-14-03-13",
language = "English",
volume = "50",
pages = "298--300",
journal = "Annali dell'Istituto Superiore di Sanita",
issn = "0021-2571",
publisher = "Istituto Superiore di Sanita",
number = "3",

}

TY - JOUR

T1 - Combined HLA matched limbal stem cells allograft with amniotic membrane transplantation as a prophylactic surgical procedure to prevent corneal graft rejection after penetrating keratoplasty

T2 - Case report

AU - Capozzi, Paolo

AU - Petroni, Sergio

AU - Buzzonetti, Luca

PY - 2014

Y1 - 2014

N2 - Purpose. To determine if the use of combined HLA matched limbal stem cells allograft with amniotic membrane transplantation (AMT) is a safe and effective prophylactic surgical procedure to prevent corneal graft after penetrating keratoplasty (PK).Methods. We report the case of a 17 years old patient with a history of congenital glaucoma, trabeculectomy and multiple corneal graft rejections, presenting total limbal cell deficiency. To reduce the possibility of graft rejection in the left eye after a new PK, a two step procedure was performed. At first the patient underwent a combined HLA matched limbal stem cells allograft (LAT) and AMT and then, 10 months later, a new PK.Results. During 12 months of follow-up, the corneal graft remained stable and smooth, with no sign of graft rejection.Conclusions. In our patient, the prophylactic use of LAT from HLA-matched donors and AMT before PK, may result in a better prognosis of corneal graft survival.

AB - Purpose. To determine if the use of combined HLA matched limbal stem cells allograft with amniotic membrane transplantation (AMT) is a safe and effective prophylactic surgical procedure to prevent corneal graft after penetrating keratoplasty (PK).Methods. We report the case of a 17 years old patient with a history of congenital glaucoma, trabeculectomy and multiple corneal graft rejections, presenting total limbal cell deficiency. To reduce the possibility of graft rejection in the left eye after a new PK, a two step procedure was performed. At first the patient underwent a combined HLA matched limbal stem cells allograft (LAT) and AMT and then, 10 months later, a new PK.Results. During 12 months of follow-up, the corneal graft remained stable and smooth, with no sign of graft rejection.Conclusions. In our patient, the prophylactic use of LAT from HLA-matched donors and AMT before PK, may result in a better prognosis of corneal graft survival.

KW - Amniotic membrane transplantation

KW - Corneal transplantation

KW - HLA matched allograft

KW - Limbal stem cells

UR - http://www.scopus.com/inward/record.url?scp=84907538804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907538804&partnerID=8YFLogxK

U2 - 10.4415/ANN-14-03-13

DO - 10.4415/ANN-14-03-13

M3 - Article

AN - SCOPUS:84907538804

VL - 50

SP - 298

EP - 300

JO - Annali dell'Istituto Superiore di Sanita

JF - Annali dell'Istituto Superiore di Sanita

SN - 0021-2571

IS - 3

ER -