Combined in utero and juvenile exposure of mice to arsenate and atrazine in drinking water modulates gene expression and clonogenicity of myeloid progenitors

Graziella Cimino-Reale, Daniele Ferrario, Barbara Casati, Roberta Brustio, Cristina Diodovich, Angelo Collotta, Marie Vahter, Laura Gribaldo

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of arsenate (As) and atrazine (Atr) on myeloid progenitors (colony-forming unit-granulocyte/macrophage, CFU-GM) cells derived from bone marrow were studied in male and female mice after combined in utero and juvenile exposure. Female adult mice were treated with arsenate in drinking water during gestation. Then, separate groups of males and females' offspring were exposed for 4 months to atrazine, to additional arsenate or to co-exposure of atrazine and arsenate together in drinking water. In male mice, arsenate and the combined exposure did not modulate the percentage of CFU-GM progenitors, whereas atrazine significantly decreases the clonogenicity of myeloid cells. In females, the percentage of CFU-GM significantly decreased after atrazine exposure did not change with arsenate treatment, but dramatically increased after the combined exposure. The expression of estrogen receptors alpha (ERα) and beta (ERβ) in bone marrow cells was investigated, and an up-regulation of receptor beta was observed in both genders. A gene expression profile was generated using nylon membranes spotted with 1185 cancer-related genes. Results from microarrays indicate that atrazine alone did not stimulate the expression of any of the genes analysed in both male and female. Arsenic induced gene expression modulation only in female. Major significant changes on the gene expression resulted following the co-exposure to arsenic and atrazine in both male and female.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalToxicology Letters
Volume180
Issue number1
DOIs
Publication statusPublished - Jul 30 2008

Keywords

  • Arsenate
  • Atrazine
  • CFU-GM
  • Estrogen receptors

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Combined in utero and juvenile exposure of mice to arsenate and atrazine in drinking water modulates gene expression and clonogenicity of myeloid progenitors'. Together they form a unique fingerprint.

Cite this