Combined label-free/fluorescence platform based on Bloch surface waves biochips for cancer biomarker detection

Norbert Danz, Alberto Sinibaldi, Matteo Allegretti, Elisabetta Sepe, Peter Munzert, Patrizio Giacomini, Francesco Michelotti

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A biosensor platform based on Bloch Surface Waves and operating in angular interrogation mode is applied to the detection of a clinical biomarker (HER2-neu/ERBB2) related to breast cancer initiation/progression. Preparing regions for specific recognition of different proteins as well as a reference on the biochip enables to correct the signal for nonspecific effects. Additionally, label-free analysis and surface wave enhanced fluorescence detection can be applied and compared directly on the platform. Cell lysates with high and low expression levels of ERBB2 are analyzed. Comparing the signals of such ERBB2 positive and negative samples estimates the limit of detection at 1.7 ng/mL. This is well below the threshold of 15 ng/mL set by the FDA for clinically useful ERBB2 detection in human serum, demonstrating that 1DPC-based biochips are attractive candidates for breast cancer detection/monitoring.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care VI
EditorsJurgen Popp, Valery V. Tuchin, Francesco Saverio Pavone
PublisherSPIE
Volume10685
ISBN (Electronic)9781510618961
DOIs
Publication statusPublished - Jan 1 2018
EventBiophotonics: Photonic Solutions for Better Health Care VI 2018 - Strasbourg, France
Duration: Apr 23 2018Apr 26 2018

Conference

ConferenceBiophotonics: Photonic Solutions for Better Health Care VI 2018
CountryFrance
CityStrasbourg
Period4/23/184/26/18

Keywords

  • 1D photonic crystals
  • Biosensor platform
  • ERBB2 tumor biomarker
  • surface wave enhanced fluorescence

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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