Abstract
The interest towards extracellular vesicles (EVs) has grown exponentially over the last few years; being involved in intercellular communication and serving as reservoirs for biomarkers for tumors, they have a great potential for liquid biopsy development, possibly replacing many costly and invasive tissue biopsies. Here we propose, for the first time, the use of a Si/SiO2 interferometric, microarray platform for multiparametric intact EVs analysis combining label-free EVs mass quantitation and high sensitivity fluorescence based phenotyping. Label free interferometric measurement allows to quantify the amount of vesicles captured by printed antibodies while, on the same chip, EVs are also detected by fluorescence in a sandwich immunoassay. The proposed method simultaneously detects, quantify and phenotype intact EVs in a microarray format.
| Original language | English |
|---|---|
| Pages (from-to) | 160-167 |
| Number of pages | 8 |
| Journal | Analytica Chimica Acta |
| Volume | 902 |
| DOIs | |
| Publication status | Published - Jan 1 2016 |
Fingerprint
Keywords
- Biomarkers
- Exosomes
- Extracellular vesicles
- Fluorescence
- Interferometry
- Microarrays
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform. / Gagni, Paola; Cretich, Marina; Benussi, Luisa; Tonoli, Elisa; Ciani, Miriam; Ghidoni, Roberta; Santini, Benedetta; Galbiati, Elisabetta; Prosperi, Davide; Chiari, Marcella.
In: Analytica Chimica Acta, Vol. 902, 01.01.2016, p. 160-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform
AU - Gagni, Paola
AU - Cretich, Marina
AU - Benussi, Luisa
AU - Tonoli, Elisa
AU - Ciani, Miriam
AU - Ghidoni, Roberta
AU - Santini, Benedetta
AU - Galbiati, Elisabetta
AU - Prosperi, Davide
AU - Chiari, Marcella
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The interest towards extracellular vesicles (EVs) has grown exponentially over the last few years; being involved in intercellular communication and serving as reservoirs for biomarkers for tumors, they have a great potential for liquid biopsy development, possibly replacing many costly and invasive tissue biopsies. Here we propose, for the first time, the use of a Si/SiO2 interferometric, microarray platform for multiparametric intact EVs analysis combining label-free EVs mass quantitation and high sensitivity fluorescence based phenotyping. Label free interferometric measurement allows to quantify the amount of vesicles captured by printed antibodies while, on the same chip, EVs are also detected by fluorescence in a sandwich immunoassay. The proposed method simultaneously detects, quantify and phenotype intact EVs in a microarray format.
AB - The interest towards extracellular vesicles (EVs) has grown exponentially over the last few years; being involved in intercellular communication and serving as reservoirs for biomarkers for tumors, they have a great potential for liquid biopsy development, possibly replacing many costly and invasive tissue biopsies. Here we propose, for the first time, the use of a Si/SiO2 interferometric, microarray platform for multiparametric intact EVs analysis combining label-free EVs mass quantitation and high sensitivity fluorescence based phenotyping. Label free interferometric measurement allows to quantify the amount of vesicles captured by printed antibodies while, on the same chip, EVs are also detected by fluorescence in a sandwich immunoassay. The proposed method simultaneously detects, quantify and phenotype intact EVs in a microarray format.
KW - Biomarkers
KW - Exosomes
KW - Extracellular vesicles
KW - Fluorescence
KW - Interferometry
KW - Microarrays
UR - http://www.scopus.com/inward/record.url?scp=84956880106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84956880106&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2015.10.017
DO - 10.1016/j.aca.2015.10.017
M3 - Article
VL - 902
SP - 160
EP - 167
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
ER -