Combined microcomputed tomography, biomechanical and histomorphometric analysis of the peri-implant bone: A pilot study in minipig model

Matteo Gramanzini, Sara Gargiulo, Fernando Zarone, Rosario Megna, Antonio Apicella, Raffaella Aversa, Marco Salvatore, Marcello Mancini, Roberto Sorrentino, Arturo Brunetti

Research output: Contribution to journalArticle

Abstract

Objectives To present a practical approach that combines biomechanical tests, microcomputed tomography (μCT) and histomorphometry, providing quantitative results on bone structure and mechanical properties in a minipig model, in order to investigate the specific response to an innovative dental biomaterial. Methods Titanium implants with innovative three-dimensional scaffolds were inserted in the tibias of 4 minipigs. Primary stability and osseointegration were investigated by means of insertion torque (IT) values, resonance frequency analysis (RFA), bone-to-implant contact (BIC), bone mineral density (BMD) and stereological measures of trabecular bone. Results A significant positive correlation was found between IT and RFA (r = 0.980, p = 0.0001). BMD at the implant sites was 18% less than the reference values (p = 0.0156). Peri-implant Tb.Th was 50% higher, while Tb.N was 50% lower than the reference zone (p <0.003) and they were negatively correlated (r = -0.897, p = 0.006). Significance μCT increases evaluation throughput and offers the possibility for qualitative three-dimensional recording of the bone-implant system as well as for non-destructive evaluation of bone architecture and mineral density, in combination with conventional analysis methods. The proposed multimodal approach allows to improve accuracy and reproducibility for peri-implant bone measurements and could support future investigations.

Original languageEnglish
Pages (from-to)794-806
Number of pages13
JournalDental Materials
Volume32
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Keywords

  • Dental implant
  • Histomorphometry
  • Insertion torque
  • Minipig model
  • Resonance frequency analysis
  • μCT

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials

Cite this

Gramanzini, M., Gargiulo, S., Zarone, F., Megna, R., Apicella, A., Aversa, R., Salvatore, M., Mancini, M., Sorrentino, R., & Brunetti, A. (2016). Combined microcomputed tomography, biomechanical and histomorphometric analysis of the peri-implant bone: A pilot study in minipig model. Dental Materials, 32(6), 794-806. https://doi.org/10.1016/j.dental.2016.03.025