Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

Marco Malavolta, Francesco Piacenza, Laura Costarelli, Robertina Giacconi, Elisa Muti, Catia Cipriano, Silvia Tesei, Sandro Spezia, Eugenio Mocchegiani

Research output: Contribution to journalArticlepeer-review

Abstract

A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount.

Original languageEnglish
Pages (from-to)1193-1198
Number of pages6
JournalJournal of Analytical Atomic Spectrometry
Volume22
Issue number9
DOIs
Publication statusPublished - 2007

ASJC Scopus subject areas

  • Spectroscopy

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