Abstract
The aim of this research was to study the influence of classic (sodium hypochlorite and chlorine dioxide) and alternative (peracetic acid [PAA]) disinfectants on the formation of mutagens in surface waters used for human consumption. For this proposal, in vivo genotoxicity tests (Comet and micronucleus assay) were performed in an experimental pilot plant set up near Lake Trasimeno (Central Italy). The effects were detected in different tissues (haemocytes for the Comet assay and gills for the micronucleus test [MN]) of Dreissena polymorpha exposed in experimental basins supplied with lake water with/without the different disinfectants. Specimen collection was performed before disinfectant input for both tests and after the start of disinfection (3 h and 20 days for the Comet assay and 10 and 20 days for micronucleus test, respectively) to assess short- and long- term exposure effects during three sampling campaigns (October 2000, February 2001, and June 2001). Seasonal differences in baseline levels of DNA migration and micronucleus frequency were observed. Raw water quality modulation on disinfection by-product formation was shown. The results of the micronucleus and Comet assays on zebra mussel cells after in situ exposure to water disinfected with the two chlorinated compounds clearly indicate DNA/by-product interaction. PAA did not induce either clastogenic/aneugenic effects or DNA damage on this bioindicator.
| Original language | English |
|---|---|
| Pages (from-to) | 127-136 |
| Number of pages | 10 |
| Journal | Science of the Total Environment |
| Volume | 333 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Oct 15 2004 |
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Keywords
- Chlorine dioxide
- DNA damage
- Dreissena polymorpha
- Peracetic acid
- Sodium hypochlorite
- Water potabilization
ASJC Scopus subject areas
- Environmental Chemistry
- Environmental Science(all)
Cite this
Comet and micronucleus assays in zebra mussel cells for genotoxicity assessment of surface drinking water treated with three different disinfectants. / Bolognesi, Claudia; Buschini, Annamaria; Branchi, Elisa; Carboni, Pamela; Furlini, Mariangela; Martino, Anna; Monteverde, Martino; Poli, Paola; Rossi, Carlo.
In: Science of the Total Environment, Vol. 333, No. 1-3, 15.10.2004, p. 127-136.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comet and micronucleus assays in zebra mussel cells for genotoxicity assessment of surface drinking water treated with three different disinfectants
AU - Bolognesi, Claudia
AU - Buschini, Annamaria
AU - Branchi, Elisa
AU - Carboni, Pamela
AU - Furlini, Mariangela
AU - Martino, Anna
AU - Monteverde, Martino
AU - Poli, Paola
AU - Rossi, Carlo
PY - 2004/10/15
Y1 - 2004/10/15
N2 - The aim of this research was to study the influence of classic (sodium hypochlorite and chlorine dioxide) and alternative (peracetic acid [PAA]) disinfectants on the formation of mutagens in surface waters used for human consumption. For this proposal, in vivo genotoxicity tests (Comet and micronucleus assay) were performed in an experimental pilot plant set up near Lake Trasimeno (Central Italy). The effects were detected in different tissues (haemocytes for the Comet assay and gills for the micronucleus test [MN]) of Dreissena polymorpha exposed in experimental basins supplied with lake water with/without the different disinfectants. Specimen collection was performed before disinfectant input for both tests and after the start of disinfection (3 h and 20 days for the Comet assay and 10 and 20 days for micronucleus test, respectively) to assess short- and long- term exposure effects during three sampling campaigns (October 2000, February 2001, and June 2001). Seasonal differences in baseline levels of DNA migration and micronucleus frequency were observed. Raw water quality modulation on disinfection by-product formation was shown. The results of the micronucleus and Comet assays on zebra mussel cells after in situ exposure to water disinfected with the two chlorinated compounds clearly indicate DNA/by-product interaction. PAA did not induce either clastogenic/aneugenic effects or DNA damage on this bioindicator.
AB - The aim of this research was to study the influence of classic (sodium hypochlorite and chlorine dioxide) and alternative (peracetic acid [PAA]) disinfectants on the formation of mutagens in surface waters used for human consumption. For this proposal, in vivo genotoxicity tests (Comet and micronucleus assay) were performed in an experimental pilot plant set up near Lake Trasimeno (Central Italy). The effects were detected in different tissues (haemocytes for the Comet assay and gills for the micronucleus test [MN]) of Dreissena polymorpha exposed in experimental basins supplied with lake water with/without the different disinfectants. Specimen collection was performed before disinfectant input for both tests and after the start of disinfection (3 h and 20 days for the Comet assay and 10 and 20 days for micronucleus test, respectively) to assess short- and long- term exposure effects during three sampling campaigns (October 2000, February 2001, and June 2001). Seasonal differences in baseline levels of DNA migration and micronucleus frequency were observed. Raw water quality modulation on disinfection by-product formation was shown. The results of the micronucleus and Comet assays on zebra mussel cells after in situ exposure to water disinfected with the two chlorinated compounds clearly indicate DNA/by-product interaction. PAA did not induce either clastogenic/aneugenic effects or DNA damage on this bioindicator.
KW - Chlorine dioxide
KW - DNA damage
KW - Dreissena polymorpha
KW - Peracetic acid
KW - Sodium hypochlorite
KW - Water potabilization
UR - http://www.scopus.com/inward/record.url?scp=4444255714&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4444255714&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2004.05.018
DO - 10.1016/j.scitotenv.2004.05.018
M3 - Article
C2 - 15364524
AN - SCOPUS:4444255714
VL - 333
SP - 127
EP - 136
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
IS - 1-3
ER -