Comet assay in cancer chemoprevention

Raffaela Santoro, Maria Ferraiuolo, Gian Paolo Morgano, Paola Muti, Sabrina Strano

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages99-105
Number of pages7
Volume1379
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1379
ISSN (Print)10643745

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Keywords

  • Comet assay
  • DNA damage
  • DNA repair
  • Double-strand breaks (DSB)
  • Genotoxic stress
  • Single-cell gel electrophoresis (SCGE)
  • Single-strand breaks (SSB)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Santoro, R., Ferraiuolo, M., Morgano, G. P., Muti, P., & Strano, S. (2016). Comet assay in cancer chemoprevention. In Methods in Molecular Biology (Vol. 1379, pp. 99-105). (Methods in Molecular Biology; Vol. 1379). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3191-0_9