TY - CHAP
T1 - Comet assay in cancer chemoprevention
AU - Santoro, Raffaela
AU - Ferraiuolo, Maria
AU - Morgano, Gian Paolo
AU - Muti, Paola
AU - Strano, Sabrina
N1 - Strano: doppia affiliazione... McMaster
PY - 2016
Y1 - 2016
N2 - The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.
AB - The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.
KW - Comet assay
KW - DNA damage
KW - DNA repair
KW - Double-strand breaks (DSB)
KW - Genotoxic stress
KW - Single-cell gel electrophoresis (SCGE)
KW - Single-strand breaks (SSB)
UR - http://www.scopus.com/inward/record.url?scp=84948455130&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84948455130&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3191-0_9
DO - 10.1007/978-1-4939-3191-0_9
M3 - Chapter
C2 - 26608293
AN - SCOPUS:84948455130
VL - 1379
T3 - Methods in Molecular Biology
SP - 99
EP - 105
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -