Comet assay in cancer chemoprevention

Raffaela Santoro, Maria Ferraiuolo, Gian Paolo Morgano, Paola Muti, Sabrina Strano

Research output: Chapter in Book/Report/Conference proceedingChapter


The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages7
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Comet assay
  • DNA damage
  • DNA repair
  • Double-strand breaks (DSB)
  • Genotoxic stress
  • Single-cell gel electrophoresis (SCGE)
  • Single-strand breaks (SSB)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Comet assay in cancer chemoprevention'. Together they form a unique fingerprint.

Cite this