Comet assay in cancer chemoprevention

Raffaela Santoro; Maria Ferraiuolo; Gian Paolo Morgano; Paola Muti; Sabrina Strano

doi:10.1007/978-1-4939-3191-0_9

Comet assay in cancer chemoprevention

Raffaela Santoro, Maria Ferraiuolo, Gian Paolo Morgano, Paola Muti, Sabrina Strano

Istituto Regina Elena

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	99-105
Number of pages	7
Volume	1379
DOIs	https://doi.org/10.1007/978-1-4939-3191-0_9
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology
Volume	1379
ISSN (Print)	10643745

Keywords

Comet assay
DNA damage
DNA repair
Double-strand breaks (DSB)
Genotoxic stress
Single-cell gel electrophoresis (SCGE)
Single-strand breaks (SSB)

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-3191-0_9

Cite this

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title = "Comet assay in cancer chemoprevention",

abstract = "The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.",

keywords = "Comet assay, DNA damage, DNA repair, Double-strand breaks (DSB), Genotoxic stress, Single-cell gel electrophoresis (SCGE), Single-strand breaks (SSB)",

author = "Raffaela Santoro and Maria Ferraiuolo and Morgano, {Gian Paolo} and Paola Muti and Sabrina Strano",

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series = "Methods in Molecular Biology",

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AU - Santoro, Raffaela

AU - Ferraiuolo, Maria

AU - Morgano, Gian Paolo

AU - Muti, Paola

AU - Strano, Sabrina

N1 - Strano: doppia affiliazione... McMaster

PY - 2016

Y1 - 2016

N2 - The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

AB - The comet assay can be useful in monitoring DNA damage in single cells caused by exposure to genotoxic agents, such as those causing air, water, and soil pollution (e.g., pesticides, dioxins, electromagnetic fields) and chemo- and radiotherapy in cancer patients, or in the assessment of genoprotective effects of chemopreventive molecules. Therefore, it has particular importance in the fields of pharmacology and toxicology, and in both environmental and human biomonitoring. It allows the detection of single strand breaks as well as double-strand breaks and can be used in both normal and cancer cells. Here we describe the alkali method for comet assay, which allows to detect both single- and double-strand DNA breaks.

KW - Comet assay

KW - DNA damage

KW - DNA repair

KW - Double-strand breaks (DSB)

KW - Genotoxic stress

KW - Single-cell gel electrophoresis (SCGE)

KW - Single-strand breaks (SSB)

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Comet assay in cancer chemoprevention

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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