Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species

Enrico Tortoli, Monica Pecorari, Giuliana Fabio, Massimino Messinò, Anna Fabio

Research output: Contribution to journalArticle

Abstract

Although commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with GenoType Mycobacterium (Hein), 317 strains, belonging to 136 species, 61 of which had never been assayed before. INNO LiPA misidentified 20 taxa, the majority of which cross-reacted with the probes specific for Mycobacterium fortuitum and the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum group. Geno-Type misidentified 28 taxa, most of which cross-reacted with M. intracellulare and M. fortuitum probes; furthermore, eight species were not recognized as members of the genus Mycobacterium. Among 54 strains investigated with AccuProbe (Gen-Probe), cross-reactions were detected for nine species, with the probes aiming at the M. avium complex being most involved in cross-reactions.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalJournal of Clinical Microbiology
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 2010

ASJC Scopus subject areas

  • Microbiology (medical)

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