Common occurrence of multiple K-RAS mutations in pancreatic cancers with associated precursor lesions and in biliary cancers

Luigi Laghi, Ombretta Orbetegli, Paolo Bianchi, Alessandro Zerbi, Valerio Di Carlo, C. Richard Boland, Alberto Malesci

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Recent studies on small series of pancreatic cancer (PC) with foci of pancreatic intraepithelial neoplasia (PanIN), a putative precursor lesion, have shown that multiple K-RAS mutations may coexist in the same neoplastic pancreas. To see whether mutant-K-RAS polyclonality is a common and specific feature of pancreatic carcinogenesis, we investigated a unselected series of periampullary cancers (41 pancreatic, 13 biliary and two ampullary adenocarcinomas). After hemi-nested polymerase chain reaction (PCR), mutations identified with single strand conformation polymorphism (SSCP) were confirmed by allele-specific PCR and sequencing. K-RAS codon 12 was mutated in 34 (83%) pancreatic cancers and in 11 (85%) biliary cancers. Multiple distinct K-RAS mutations were found in 16 PC (39% of all cases, 47% of those with mutated K-RAS) and in eight biliary cancers (62 and 72%, respectively). In PC, multiple K-RAS mutations were more frequent (P

Original languageEnglish
Pages (from-to)4301-4306
Number of pages6
JournalOncogene
Volume21
Issue number27
DOIs
Publication statusPublished - Jun 20 2002

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Biliary Tract Neoplasms
Pancreatic Neoplasms
Mutation
Polymerase Chain Reaction
Codon
Pancreas
Carcinogenesis
Adenocarcinoma
Alleles
Neoplasms

Keywords

  • Bile duct cancer
  • K-RAS
  • Pancreatic cancer
  • Pancreatic intraepithelial neoplasia

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Common occurrence of multiple K-RAS mutations in pancreatic cancers with associated precursor lesions and in biliary cancers. / Laghi, Luigi; Orbetegli, Ombretta; Bianchi, Paolo; Zerbi, Alessandro; Di Carlo, Valerio; Boland, C. Richard; Malesci, Alberto.

In: Oncogene, Vol. 21, No. 27, 20.06.2002, p. 4301-4306.

Research output: Contribution to journalArticle

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