Comparative analysis between saliva and buccal swabs as source of DNA: Lesson from HLA-B∗57:01 testing

Raffaella Cascella, Laura Stocchi, Claudia Strafella, Ivano Mezzaroma, Marco Mannazzu, Vincenzo Vullo, Francesco Montella, Giustino Parruti, Paola Borgiani, Federica Sangiuolo, Giuseppe Novelli, Antonella Pirazzoli, Stefania Zampatti, Emiliano Giardina

Research output: Contribution to journalArticlepeer-review

Abstract

Our work aimed to designate the optimal DNA source for pharmacogenetic assays, such as the screening for HLA-B∗57:01 allele. Materials and methods: A saliva and four buccal swab samples were taken from 104 patients. All the samples were stored at different time and temperature conditions and then genotyped for the HLA-B∗57:01 allele by SSP-PCR and classical/capillary electrophoresis. Results: The genotyping analysis reported different performance rates depending on the storage conditions of the samples. Given our results, the buccal swab demonstrated to be more resistant and stable in time with respect to the saliva. Conclusion: Our investigation designates the buccal swab as the optimal DNA source for pharmacogenetic assays in terms of resistance, low infectivity, low-invasiveness and easy sampling, and safe transport in centralized medical centers providing specialized pharmacogenetic tests.

Original languageEnglish
Pages (from-to)1039-1046
Number of pages8
JournalPharmacogenomics
Volume16
Issue number10
DOIs
Publication statusPublished - Jul 1 2015

Keywords

  • DNA source
  • HLA-B∗57:01 testing
  • hypersensitivity reaction
  • molecular assay
  • personalized medicine
  • pharmacogenetics

ASJC Scopus subject areas

  • Pharmacology
  • Genetics
  • Molecular Medicine

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