Abstract
Objective: Our purpose was to compare the performance of 2 recently introduced molecular tests for the identification of gastrointestinal viral infections. Methods: One hundred fecal samples from pediatric patients were analyzed using 2 workflows, each including nucleic acids extraction and multiplex Real-Time PCR: Allplex™ GI-Virus Assay and FTD Viral gastroenteritis. The agreement was evaluated calculating Cohen's kappa and applying McNemar's test. Results and conclusion: Allplex and FTD assays showed 100% overall agreement for Norovirus GI/GII and Sapovirus (κ: 1.00), and 99% for Astrovirus (κ: 0.66). A lower agreement was detected for Adenovirus (89%; κ: 0.72) and Rotavirus (91%, k: 0.53), owing to samples resulted positive only with FTD test. The discrepancies were attributed to a different efficiency of extraction/amplification and to the different Adenovirus serotype specificity of the tests since Allplex detects only AdVF40 and AdVF41. FTD test should be used when non enteric adenovirus could have a clinical significance.
| Original language | English |
|---|---|
| Article number | 114893 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 96 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2020 |
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Keywords
- Enteric viruses
- Gastroenteritis
- Molecular diagnostic
- Multiplex real-time PCR
- Syndromic testing
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
Cite this
Comparative analysis of 2 commercial molecular tests for the detection of gastroenteric viruses on stool samples. / Sciandra, I.; Piccioni, L.; Coltella, L.; Ranno, S.; Giannelli, G.; Falasca, F.; Antonelli, G.; Concato, C.; Turriziani, O.
In: Diagnostic Microbiology and Infectious Disease, Vol. 96, No. 1, 114893, 01.2020.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative analysis of 2 commercial molecular tests for the detection of gastroenteric viruses on stool samples
AU - Sciandra, I.
AU - Piccioni, L.
AU - Coltella, L.
AU - Ranno, S.
AU - Giannelli, G.
AU - Falasca, F.
AU - Antonelli, G.
AU - Concato, C.
AU - Turriziani, O.
PY - 2020/1
Y1 - 2020/1
N2 - Objective: Our purpose was to compare the performance of 2 recently introduced molecular tests for the identification of gastrointestinal viral infections. Methods: One hundred fecal samples from pediatric patients were analyzed using 2 workflows, each including nucleic acids extraction and multiplex Real-Time PCR: Allplex™ GI-Virus Assay and FTD Viral gastroenteritis. The agreement was evaluated calculating Cohen's kappa and applying McNemar's test. Results and conclusion: Allplex and FTD assays showed 100% overall agreement for Norovirus GI/GII and Sapovirus (κ: 1.00), and 99% for Astrovirus (κ: 0.66). A lower agreement was detected for Adenovirus (89%; κ: 0.72) and Rotavirus (91%, k: 0.53), owing to samples resulted positive only with FTD test. The discrepancies were attributed to a different efficiency of extraction/amplification and to the different Adenovirus serotype specificity of the tests since Allplex detects only AdVF40 and AdVF41. FTD test should be used when non enteric adenovirus could have a clinical significance.
AB - Objective: Our purpose was to compare the performance of 2 recently introduced molecular tests for the identification of gastrointestinal viral infections. Methods: One hundred fecal samples from pediatric patients were analyzed using 2 workflows, each including nucleic acids extraction and multiplex Real-Time PCR: Allplex™ GI-Virus Assay and FTD Viral gastroenteritis. The agreement was evaluated calculating Cohen's kappa and applying McNemar's test. Results and conclusion: Allplex and FTD assays showed 100% overall agreement for Norovirus GI/GII and Sapovirus (κ: 1.00), and 99% for Astrovirus (κ: 0.66). A lower agreement was detected for Adenovirus (89%; κ: 0.72) and Rotavirus (91%, k: 0.53), owing to samples resulted positive only with FTD test. The discrepancies were attributed to a different efficiency of extraction/amplification and to the different Adenovirus serotype specificity of the tests since Allplex detects only AdVF40 and AdVF41. FTD test should be used when non enteric adenovirus could have a clinical significance.
KW - Enteric viruses
KW - Gastroenteritis
KW - Molecular diagnostic
KW - Multiplex real-time PCR
KW - Syndromic testing
UR - http://www.scopus.com/inward/record.url?scp=85074504841&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85074504841&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2019.114893
DO - 10.1016/j.diagmicrobio.2019.114893
M3 - Article
C2 - 31677959
AN - SCOPUS:85074504841
VL - 96
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 1
M1 - 114893
ER -