Comparative analysis of 2 commercial molecular tests for the detection of gastroenteric viruses on stool samples

I. Sciandra, L. Piccioni, L. Coltella, S. Ranno, G. Giannelli, F. Falasca, G. Antonelli, C. Concato, O. Turriziani

Research output: Contribution to journalArticle

Abstract

Objective: Our purpose was to compare the performance of 2 recently introduced molecular tests for the identification of gastrointestinal viral infections. Methods: One hundred fecal samples from pediatric patients were analyzed using 2 workflows, each including nucleic acids extraction and multiplex Real-Time PCR: Allplex™ GI-Virus Assay and FTD Viral gastroenteritis. The agreement was evaluated calculating Cohen's kappa and applying McNemar's test. Results and conclusion: Allplex and FTD assays showed 100% overall agreement for Norovirus GI/GII and Sapovirus (κ: 1.00), and 99% for Astrovirus (κ: 0.66). A lower agreement was detected for Adenovirus (89%; κ: 0.72) and Rotavirus (91%, k: 0.53), owing to samples resulted positive only with FTD test. The discrepancies were attributed to a different efficiency of extraction/amplification and to the different Adenovirus serotype specificity of the tests since Allplex detects only AdVF40 and AdVF41. FTD test should be used when non enteric adenovirus could have a clinical significance.

Original languageEnglish
Article number114893
JournalDiagnostic Microbiology and Infectious Disease
Volume96
Issue number1
DOIs
Publication statusPublished - Jan 2020

Fingerprint

Adenoviridae
Viruses
Sapovirus
Norovirus
Workflow
Rotavirus
Multiplex Polymerase Chain Reaction
Gastroenteritis
Virus Diseases
Nucleic Acids
Real-Time Polymerase Chain Reaction
Pediatrics

Keywords

  • Enteric viruses
  • Gastroenteritis
  • Molecular diagnostic
  • Multiplex real-time PCR
  • Syndromic testing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Comparative analysis of 2 commercial molecular tests for the detection of gastroenteric viruses on stool samples. / Sciandra, I.; Piccioni, L.; Coltella, L.; Ranno, S.; Giannelli, G.; Falasca, F.; Antonelli, G.; Concato, C.; Turriziani, O.

In: Diagnostic Microbiology and Infectious Disease, Vol. 96, No. 1, 114893, 01.2020.

Research output: Contribution to journalArticle

Sciandra, I. ; Piccioni, L. ; Coltella, L. ; Ranno, S. ; Giannelli, G. ; Falasca, F. ; Antonelli, G. ; Concato, C. ; Turriziani, O. / Comparative analysis of 2 commercial molecular tests for the detection of gastroenteric viruses on stool samples. In: Diagnostic Microbiology and Infectious Disease. 2020 ; Vol. 96, No. 1.
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