Comparative analysis of antibody and cell-mediated autoimmunity to transaldolase and myelin basic protein in patients with multiple sclerosis

Emanuela Colombo, Katalin Banki, Arthur H. Tatum, John Daucher, Pasquale Ferrante, Ronald S. Murray, Paul E. Phillips, Andras Perl

Research output: Contribution to journalArticlepeer-review

Abstract

Antibody and T cell-mediated immune responses to oligodendroglial autoantigens transaldolase (TAL) and myelin basic protein (MBP) were examined in patients with multiple sclerosis (MS). Immunohistochemical studies of postmortem brain sections revealed decreased staining by MBP- and TAL- specific antibodies in MS plaques, indicating a concurrent loss of these antigens from demyelination sites. By Western blot high titer antibodies to human recombinant TAL were found in 29/94 sera and 16/23 cerebrospinal fluid samples from MS patients. Antibodies to MBP were undetectable in sera or cerebrospinal fluid of these MS patients. Proliferative responses to human recombinant TAL (stimulation index [SI] = 2.47±0.3) were significantly increased in comparison to MBP in 25 patients with MS (SI 1.37±0.1; P <0.01). After a 7-d stimulation of PBL, utilization of any of 24 different T cell receptor Vβ gene segments in response to MBP was increased less than twofold in the two control donors and six MS patients investigated. In response to TAL-H, while skewing of individual Vβ genes was also less than twofold in healthy controls, usage of specific Vβ gene segments was differentially increased ranging from 2.5 to 65.9-fold in patients with MS. The results suggest that TAL may be a more potent immunogen than MBP in MS.

Original languageEnglish
Pages (from-to)1238-1250
Number of pages13
JournalJournal of Clinical Investigation
Volume99
Issue number6
Publication statusPublished - Mar 15 1997

Keywords

  • multiple sclerosis
  • myelin basic protein
  • transaldolase

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Comparative analysis of antibody and cell-mediated autoimmunity to transaldolase and myelin basic protein in patients with multiple sclerosis'. Together they form a unique fingerprint.

Cite this