Comparative analysis of different approaches to investigate cell kinetics

R. Silvestrini, A. Costa, S. Veneroni, G. Del Bino, P. Persici

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The potential of different methods to investigate proliferative activity of cell populations was analysed for non-Hodgkin's lymphomas. Cells in S phase and all cycling cells were determined on cell suspensions obtained from fresh lymph node material by [3H]-thymidine autoradiography ([3H]TdR LI), a monoclonal antibody to bromodeoxyuridine (BrdU LI), and the monoclonal antibody Ki67. A good correlation was observed between the values of [3H]TdR LI and BrdU LI (r(s) = 0.90; P <0.01), [3H]TdR LI and S phase (r(s) = 0.62; P <0.01) and [3H]TdR LI and Ki67 (r(s) = 0.64; P <0.01) in individual lymphomas. Using the median values obtained from the different approaches as cut-off points to define slowly and rapidly proliferating tumours, the best agreement was observed between [3H]TdR LI and BrdU LI (91%) and poorer agreements, even though statistically significant, were observed between [3H]TdR LI and S phase (73%) or Ki67 (76%). In conclusion, the kinetic information derived from different approaches was more or less concordant and newly proposed approaches should be directly and carefully verified for their prognostic relevance before using them as alternative to conventional methods.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalCell and Tissue Kinetics
Issue number2
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Cell Biology


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