Comparative analysis of different methods to detect Clostridium difficile infection

Adriana Calderaro, Mirko Buttrini, Monica Martinelli, Chiara Gorrini, Sara Montecchini, Maria Cristina Medici, Maria Cristina Arcangeletti, Flora De Conto, Silvia Covan, Carlo Chezzi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the IllumigeneTM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100%. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57%. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalNew Microbiologica
Volume36
Issue number1
Publication statusPublished - 2013

Fingerprint

Clostridium Infections
Clostridium difficile
Immunochromatography
North America
Prospective Studies
Costs and Cost Analysis
DNA
Incidence

Keywords

  • Clostridium difficile
  • Diagnosis
  • Diarrhoea
  • LAMP
  • Toxins

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Calderaro, A., Buttrini, M., Martinelli, M., Gorrini, C., Montecchini, S., Medici, M. C., ... Chezzi, C. (2013). Comparative analysis of different methods to detect Clostridium difficile infection. New Microbiologica, 36(1), 57-63.

Comparative analysis of different methods to detect Clostridium difficile infection. / Calderaro, Adriana; Buttrini, Mirko; Martinelli, Monica; Gorrini, Chiara; Montecchini, Sara; Medici, Maria Cristina; Arcangeletti, Maria Cristina; De Conto, Flora; Covan, Silvia; Chezzi, Carlo.

In: New Microbiologica, Vol. 36, No. 1, 2013, p. 57-63.

Research output: Contribution to journalArticle

Calderaro, A, Buttrini, M, Martinelli, M, Gorrini, C, Montecchini, S, Medici, MC, Arcangeletti, MC, De Conto, F, Covan, S & Chezzi, C 2013, 'Comparative analysis of different methods to detect Clostridium difficile infection', New Microbiologica, vol. 36, no. 1, pp. 57-63.
Calderaro A, Buttrini M, Martinelli M, Gorrini C, Montecchini S, Medici MC et al. Comparative analysis of different methods to detect Clostridium difficile infection. New Microbiologica. 2013;36(1):57-63.
Calderaro, Adriana ; Buttrini, Mirko ; Martinelli, Monica ; Gorrini, Chiara ; Montecchini, Sara ; Medici, Maria Cristina ; Arcangeletti, Maria Cristina ; De Conto, Flora ; Covan, Silvia ; Chezzi, Carlo. / Comparative analysis of different methods to detect Clostridium difficile infection. In: New Microbiologica. 2013 ; Vol. 36, No. 1. pp. 57-63.
@article{168ce8fd712e4d72b23041ef76e0c2e7,
title = "Comparative analysis of different methods to detect Clostridium difficile infection",
abstract = "The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the IllumigeneTM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100{\%}. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57{\%}. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample.",
keywords = "Clostridium difficile, Diagnosis, Diarrhoea, LAMP, Toxins",
author = "Adriana Calderaro and Mirko Buttrini and Monica Martinelli and Chiara Gorrini and Sara Montecchini and Medici, {Maria Cristina} and Arcangeletti, {Maria Cristina} and {De Conto}, Flora and Silvia Covan and Carlo Chezzi",
year = "2013",
language = "English",
volume = "36",
pages = "57--63",
journal = "New Microbiologica",
issn = "1121-7138",
publisher = "Luigi Ponzio e figlio Editori",
number = "1",

}

TY - JOUR

T1 - Comparative analysis of different methods to detect Clostridium difficile infection

AU - Calderaro, Adriana

AU - Buttrini, Mirko

AU - Martinelli, Monica

AU - Gorrini, Chiara

AU - Montecchini, Sara

AU - Medici, Maria Cristina

AU - Arcangeletti, Maria Cristina

AU - De Conto, Flora

AU - Covan, Silvia

AU - Chezzi, Carlo

PY - 2013

Y1 - 2013

N2 - The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the IllumigeneTM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100%. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57%. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample.

AB - The increased incidence and severity of Clostridium difficile infection, particularly in North America and Europe, have brought renewed focus on the most appropriate method to detect C. difficile and/or its toxins in stools. This prospective study evaluated the usefulness of the IllumigeneTM C. difficile assay in diagnostic practice for the detection of toxigenic C. difficile DNA in clinical samples. A total of 88 out of 306 stool samples analysed were positive both by Illumigene and the combination of toxigenic C. difficile culture (TC) and immunochromatographic assay (IC) with a concordance of 100%. Of the 218 samples negative by the combination of TC and IC, 204 were negative also by Illumigene with a concordance of 93.57%. In our experience, compared to conventional assays Illumigene assay proved to be easy to perform, accurate and prompt giving results within 1 hour at a cost of 28 euro per sample.

KW - Clostridium difficile

KW - Diagnosis

KW - Diarrhoea

KW - LAMP

KW - Toxins

UR - http://www.scopus.com/inward/record.url?scp=84874619808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874619808&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 57

EP - 63

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

IS - 1

ER -