Comparative analysis of immune responses and cytokine profiles elicited in rabbits by the combined use of recombinant fowlpox viruses, plasmids and virus-like particles in prime-boost vaccination protocols against SHIV

Antonia Radaelli, Carlo Zanotto, Gianpaolo Perletti, Veronica Elli, Elisa Vicenzi, Guido Poli, Carlo De Giuli Morghen

Research output: Contribution to journalArticlepeer-review

Abstract

Three different prime-boost immunization protocols were tested in rabbits and their immune response was evaluated and compared with the final aim of identifying a vaccine strategy that might be able to protect non-human primates from infection with the pathogenic chimera simian/human immunodeficiency virus (SHIV)89.6P. Protocols were based on priming with two fowlpox (FP) recombinant vectors and two expression plasmids, which express either the simian immunodeficiency virus (SIV)mac239 gag/pol or the human immunodeficiency virus (HIV-1)env89.6P genes, followed by boosting with virus-like particles (VLP). All protocols were effective in eliciting homologous neutralizing Ab and highlighted the efficacy of VLP boosting. The FP vector was less efficient than plasmid DNA in inducing Ab against the gag core proteins. Analysis of cytokine expression 5 months after last immunization indicated that priming with pcDNA3gag/polSIV and FPenv89.6P followed by VLP boosting generated a T helper (Th0) profile and a good Ab titer, suggesting a potential protocol to be tested in the SHIV-macaque model of HIV-1 infection.

Original languageEnglish
Pages (from-to)2061-2073
Number of pages13
JournalVaccine
Volume21
Issue number17-18
DOIs
Publication statusPublished - May 16 2003

Keywords

  • Cytokines
  • Fowlpox virus
  • Prime-boost
  • Recombinant vaccines
  • VLP

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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