Abstract
A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.
| Original language | English |
|---|---|
| Pages (from-to) | 1993-2000 |
| Number of pages | 8 |
| Journal | Vaccine |
| Volume | 25 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Mar 1 2007 |
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Keywords
- Filamentous bacteriophage fd
- Macromolecular scaffolds
- Pyruvate dehydrogenase
ASJC Scopus subject areas
- Immunology
- Microbiology
- Virology
- Infectious Diseases
- Public Health, Environmental and Occupational Health
- veterinary(all)
Cite this
Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems. / D'Apice, Luciana; Sartorius, Rossella; Caivano, Antonella; Mascolo, Dina; Del Pozzo, Giovanna; Di Mase, Donatella Scotto; Ricca, Ezio; Pira, Giuseppina Li; Manca, Fabrizio; Malanga, Donatella; De Palma, Raffaele; De Berardinis, Piergiuseppe.
In: Vaccine, Vol. 25, No. 11, 01.03.2007, p. 1993-2000.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems
AU - D'Apice, Luciana
AU - Sartorius, Rossella
AU - Caivano, Antonella
AU - Mascolo, Dina
AU - Del Pozzo, Giovanna
AU - Di Mase, Donatella Scotto
AU - Ricca, Ezio
AU - Pira, Giuseppina Li
AU - Manca, Fabrizio
AU - Malanga, Donatella
AU - De Palma, Raffaele
AU - De Berardinis, Piergiuseppe
PY - 2007/3/1
Y1 - 2007/3/1
N2 - A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.
AB - A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.
KW - Filamentous bacteriophage fd
KW - Macromolecular scaffolds
KW - Pyruvate dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=33846929623&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846929623&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2006.11.047
DO - 10.1016/j.vaccine.2006.11.047
M3 - Article
C2 - 17239998
AN - SCOPUS:33846929623
VL - 25
SP - 1993
EP - 2000
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 11
ER -