Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Luciana D'Apice; Rossella Sartorius; Antonella Caivano; Dina Mascolo; Giovanna Del Pozzo; Donatella Scotto Di Mase; Ezio Ricca; Giuseppina Li Pira; Fabrizio Manca; Donatella Malanga; Raffaele De Palma; Piergiuseppe De Berardinis

doi:10.1016/j.vaccine.2006.11.047

Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Luciana D'Apice, Rossella Sartorius, Antonella Caivano, Dina Mascolo, Giovanna Del Pozzo, Donatella Scotto Di Mase, Ezio Ricca, Giuseppina Li Pira, Fabrizio Manca, Donatella Malanga, Raffaele De Palma, Piergiuseppe De Berardinis

Research output: Contribution to journal › Article › peer-review

Abstract

A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.

Original language	English
Pages (from-to)	1993-2000
Number of pages	8
Journal	Vaccine
Volume	25
Issue number	11
DOIs	https://doi.org/10.1016/j.vaccine.2006.11.047
Publication status	Published - Mar 1 2007

Keywords

Filamentous bacteriophage fd
Macromolecular scaffolds
Pyruvate dehydrogenase

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

Access to Document

10.1016/j.vaccine.2006.11.047

Fingerprint Dive into the research topics of 'Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems'. Together they form a unique fingerprint.

Cite this

Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems. / D'Apice, Luciana; Sartorius, Rossella; Caivano, Antonella; Mascolo, Dina; Del Pozzo, Giovanna; Di Mase, Donatella Scotto; Ricca, Ezio; Pira, Giuseppina Li; Manca, Fabrizio; Malanga, Donatella; De Palma, Raffaele; De Berardinis, Piergiuseppe.

In: Vaccine, Vol. 25, No. 11, 01.03.2007, p. 1993-2000.

Research output: Contribution to journal › Article › peer-review

D'Apice, Luciana ; Sartorius, Rossella ; Caivano, Antonella ; Mascolo, Dina ; Del Pozzo, Giovanna ; Di Mase, Donatella Scotto ; Ricca, Ezio ; Pira, Giuseppina Li ; Manca, Fabrizio ; Malanga, Donatella ; De Palma, Raffaele ; De Berardinis, Piergiuseppe. / Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems. In: Vaccine. 2007 ; Vol. 25, No. 11. pp. 1993-2000.

@article{8249254afe7f4c75a36c233768dd8f98,

title = "Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems",

abstract = "A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.",

keywords = "Filamentous bacteriophage fd, Macromolecular scaffolds, Pyruvate dehydrogenase",

author = "Luciana D'Apice and Rossella Sartorius and Antonella Caivano and Dina Mascolo and {Del Pozzo}, Giovanna and {Di Mase}, {Donatella Scotto} and Ezio Ricca and Pira, {Giuseppina Li} and Fabrizio Manca and Donatella Malanga and {De Palma}, Raffaele and {De Berardinis}, Piergiuseppe",

year = "2007",

month = mar,

day = "1",

doi = "10.1016/j.vaccine.2006.11.047",

language = "English",

volume = "25",

pages = "1993--2000",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "11",

}

TY - JOUR

T1 - Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

AU - D'Apice, Luciana

AU - Sartorius, Rossella

AU - Caivano, Antonella

AU - Mascolo, Dina

AU - Del Pozzo, Giovanna

AU - Di Mase, Donatella Scotto

AU - Ricca, Ezio

AU - Pira, Giuseppina Li

AU - Manca, Fabrizio

AU - Malanga, Donatella

AU - De Palma, Raffaele

AU - De Berardinis, Piergiuseppe

PY - 2007/3/1

Y1 - 2007/3/1

N2 - A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.

AB - A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.

KW - Filamentous bacteriophage fd

KW - Macromolecular scaffolds

KW - Pyruvate dehydrogenase

UR - http://www.scopus.com/inward/record.url?scp=33846929623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846929623&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2006.11.047

DO - 10.1016/j.vaccine.2006.11.047

M3 - Article

C2 - 17239998

AN - SCOPUS:33846929623

VL - 25

SP - 1993

EP - 2000

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 11

ER -