Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Luciana D'Apice, Rossella Sartorius, Antonella Caivano, Dina Mascolo, Giovanna Del Pozzo, Donatella Scotto Di Mase, Ezio Ricca, Giuseppina Li Pira, Fabrizio Manca, Donatella Malanga, Raffaele De Palma, Piergiuseppe De Berardinis

Research output: Contribution to journalArticlepeer-review

Abstract

A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.

Original languageEnglish
Pages (from-to)1993-2000
Number of pages8
JournalVaccine
Volume25
Issue number11
DOIs
Publication statusPublished - Mar 1 2007

Keywords

  • Filamentous bacteriophage fd
  • Macromolecular scaffolds
  • Pyruvate dehydrogenase

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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