Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Luciana D'Apice; Rossella Sartorius; Antonella Caivano; Dina Mascolo; Giovanna Del Pozzo; Donatella Scotto Di Mase; Ezio Ricca; Giuseppina Li Pira; Fabrizio Manca; Donatella Malanga; Raffaele De Palma; Piergiuseppe De Berardinis

doi:10.1016/j.vaccine.2006.11.047

Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Luciana D'Apice, Rossella Sartorius, Antonella Caivano, Dina Mascolo, Giovanna Del Pozzo, Donatella Scotto Di Mase, Ezio Ricca, Giuseppina Li Pira, Fabrizio Manca, Donatella Malanga, Raffaele De Palma, Piergiuseppe De Berardinis

Research output: Contribution to journal › Article › peer-review

Abstract

A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.

Original language	English
Pages (from-to)	1993-2000
Number of pages	8
Journal	Vaccine
Volume	25
Issue number	11
DOIs	https://doi.org/10.1016/j.vaccine.2006.11.047
Publication status	Published - Mar 1 2007

Keywords

Filamentous bacteriophage fd
Macromolecular scaffolds
Pyruvate dehydrogenase

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

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10.1016/j.vaccine.2006.11.047

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Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems. / D'Apice, Luciana; Sartorius, Rossella; Caivano, Antonella; Mascolo, Dina; Del Pozzo, Giovanna; Di Mase, Donatella Scotto; Ricca, Ezio; Pira, Giuseppina Li; Manca, Fabrizio; Malanga, Donatella; De Palma, Raffaele; De Berardinis, Piergiuseppe.

In: Vaccine, Vol. 25, No. 11, 01.03.2007, p. 1993-2000.

Research output: Contribution to journal › Article › peer-review

D'Apice, Luciana ; Sartorius, Rossella ; Caivano, Antonella ; Mascolo, Dina ; Del Pozzo, Giovanna ; Di Mase, Donatella Scotto ; Ricca, Ezio ; Pira, Giuseppina Li ; Manca, Fabrizio ; Malanga, Donatella ; De Palma, Raffaele ; De Berardinis, Piergiuseppe. / Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems. In: Vaccine. 2007 ; Vol. 25, No. 11. pp. 1993-2000.

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abstract = "A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system.",

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AU - Del Pozzo, Giovanna

AU - Di Mase, Donatella Scotto

AU - Ricca, Ezio

AU - Pira, Giuseppina Li

AU - Manca, Fabrizio

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Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems

Abstract

Keywords

ASJC Scopus subject areas

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