Comparative analysis of plant-produced, recombinant dimeric IgA against cell wall β-glucan of pathogenic fungi

Cristina Capodicasa, Marcello Catellani, Ilaria Moscetti, Carla Bromuro, Paola Chiani, Antonella Torosantucci, Eugenio Benvenuto

Research output: Contribution to journalArticlepeer-review

Abstract

Immunoglobulins A (IgA) are crucially involved in protection of human mucosal surfaces from microbial pathogens. In this work, we devised and expressed in plants recombinant chimeric antifungal antibodies (Abs) of isotype A (IgA1, IgA2, and scFvFcA1), derived from a murine mAb directed to the fungal cell wall polysaccharide β-glucan which had proven able to confer protection against multiple pathogenic fungi. All recombinant IgA (rIgA) were expressed and correctly assembled in dimeric form in plants and evaluated for yield, antigen-binding efficiency and antifungal properties in vitro, in comparison with a chimeric IgG1 version. Production yields and binding efficiency to purified β-glucans showed significant variations not only between Abs of different isotypes but also between the different IgA formats. Moreover, only the dimeric IgA1 was able to strongly bind cells of the fungal pathogen Candida albicans and to restrain its adhesion to human epithelial cells. Our data indicate that IgG to IgA switch and differences in molecular structure among different rIgA formats can impact expression in plant and biological activity of anti-β-glucans Abs and provide new insights for the design of recombinant IgA as anti-infective immunotherapeutics, whose potential is still poorly investigated.

Original languageEnglish
Pages (from-to)2729-2738
Number of pages10
JournalBiotechnology and Bioengineering
Volume114
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

Keywords

  • anti-infectious IgA
  • antigen binding efficiency
  • fungal infections
  • immunotherapy
  • plant pharming
  • recombinant IgA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Comparative analysis of plant-produced, recombinant dimeric IgA against cell wall β-glucan of pathogenic fungi'. Together they form a unique fingerprint.

Cite this