Comparative analysis of proliferating cell nuclear antigen and epidermal growth factor receptor expression in glial tumours: Correlation with histological grading

Marcella Mottolese, Pier Giorgio Natali, Antonella Coli, Giulio Bigotti, Maria Benevolo, Antonio Cione, Laura Raus, Carmine M. Carapella

Research output: Contribution to journalArticle

Abstract

The present study analysed the combined immunostaining for proliferating cell nuclear antigen (PCNA) and epidermal growth factor receptor (EGFR) with the aim of obtaining an objective method for the evaluation of the growth fraction in human glial tumours. A retrospective study was undertaken on 157 gliomas employing MoAb PC1O and MoAb 108, recognising a 36 Kd nuclear protein associated with the cell cycle and the extracellular domain of the EGFR, respectively. The results of this immunohistochemical analysis showed that the rate of PCNA positive cell is directly associated to EGFR expression and significantly (P <0.0001) correlates with tumour morphological grading, this was also the case in patients submitted to multiple surgical treatments for recurrent tumours. PCNA and/or EGFR are expressed by a minority of low grade astrocytoma, while anaplastic astrocytoma and glioblastoma displayed an intense immunoreactivity for the two antigens in more than 85% of tested cases. These findings indicate that the combined evaluation of PCNA and EGFR could allow a more definite biopathological grading of neuroepithelial brain tumours.

Original languageEnglish
Pages (from-to)1951-1956
Number of pages6
JournalAnticancer Research
Volume18
Issue number3 B
Publication statusPublished - 1998

Keywords

  • Epidermal growth factor receptor
  • Glioma
  • Immunohistochemistry
  • Malignancy grading
  • Proliferating cell nuclear antigen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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