Comparative analysis of proneural gene expression in the embryonic cerebellum

Paola Zordan, Laura Croci, Richard Hawkes, G. Giacomo Consalez

Research output: Contribution to journalArticlepeer-review

Abstract

The embryonic cerebellum contains two germinative epithelia: the rhombic lip and the ventricular zone. While the lineage of glutamatergic neurons arising from the rhombic lip has been characterized, plenty remains to be learned about the factors giving rise to the array of ventricular zone-derived γ-aminobutyric acid (GABA)ergic neurons. In the present study, we describe the expression of proneural genes Mash1/Ascl1, Ngn1/Neurog1, and Ngn2/Neurog2 in the cerebellar primordium at key stages of Purkinje cell and interneuron development, and compare them with the expression of other genes active in the same context. Our results indicate that Ngn1, Ngn2 and Mash1 are expressed at relevant stages of cerebellar neurogenesis in the prospective cerebellar nuclei and in the ventricular zone, excluding the Math1/Atoh1-positive rhombic lip. Their expression domains are only partially overlapping, suggesting that they may contribute selectively to ventricular zone regionalization, giving rise to the diversity of cerebellar GABA neurons and, possibly, Purkinje cell subtypes.

Original languageEnglish
Pages (from-to)1726-1735
Number of pages10
JournalDevelopmental Dynamics
Volume237
Issue number6
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Ascl1, neurog1
  • Cell fate specification
  • Cerebellar development
  • Cerebellum
  • GABAergic
  • Neurog2
  • Neurogenesis
  • Neurogenin
  • Purkinje neurons
  • Regionalization

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Fingerprint

Dive into the research topics of 'Comparative analysis of proneural gene expression in the embryonic cerebellum'. Together they form a unique fingerprint.

Cite this