Comparative effects of Aβ(1-42)-Al complex from rat and human amyloid on rat endothelial cell cultures

Denise Drago, Marcella Folin, Silvia Baiguera, Giuseppe Tognon, Fernanda Ricchelli, Paolo Zatta

Research output: Contribution to journalArticlepeer-review

Abstract

Metal ions are widely recognized as a key factor for the conformational changes and aggregation of the Alzheimer's disease amyloid (Aβ). So far Al3+ has received much less attention than other biometals in terms of interaction with Aβ. Brain endothelial cells have been identified as important regulators of the neuronal microenvironment, including Aβ levels. The purpose of this study is to compare the effects of the complex amyloid (Aβ1-42)-Al, from human and rat, with the effects produced by metal-free Aβ on rat neuroendothelial cells (NECs). To establish Aβ and Aβ-Al toxicity on NECs, survival, vitality, and angiogenesis are evaluated. Cell survival is reduced by human and rat Aβ in a time-dependent manner. This toxic effect is remarkably pronounced in the presence of human Aβ-Al. Moreover, rat Aβ has anti-angiogenic properties on NECs, and this effect is aggravated dramatically by using both human and rat Aβ-Al complexes. The data and arguments presented herein clearly demonstrate the involvement of Al3+ in Aβ aggregation and, consequently, increasing endothelial cell toxicity.

Original languageEnglish
Pages (from-to)33-44
Number of pages12
JournalJournal of Alzheimer's Disease
Volume11
Issue number1
Publication statusPublished - 2007

Keywords

  • Aluminum
  • Alzheimer
  • Amyloid
  • Blood-brain barrier
  • Endothelial cells
  • Metal ions

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology

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