Comparative evaluation of a commercial enzyme-linked immunoassay and solid-phase immune electron microscopy for rotavirus detection in stool specimens

G. Gerna, A. Sarasini, N. Passarani, M. Torsellini, M. Parea, M. Battaglia

Research output: Contribution to journalArticle

Abstract

Using solid-phase immune electron microscopy (SPIEM) as a reference test, we examined 151 stool specimens from infants and young children with acute gastroenteritis for rotavirus detection by a one-step commercial enzyme-linked immunosorbent assay (ELISA) with labeled monoclonal antibody. Of the 83 samples determined to be positive for rotavirus by SPIEM, 82 were detected as positive by the monoclonal antibody ELISA (sensitivity, 98.7%), while 67 of the 68 specimens determined to be negative by SPIEM were correctly detected as negative by the ELISA (specificity, 98.5%). The diagnostic accuracy of the ELISA kit was 98.6%. Thus, the one-step monoclonal antibody ELISA, which can be completed in less than 90 min, appears to be highly suitable for the rapid and reliable detection of rotavirus in stools.

Original languageEnglish
Pages (from-to)1137-1139
Number of pages3
JournalJournal of Clinical Microbiology
Volume25
Issue number6
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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