TY - JOUR
T1 - Comparative evaluation of nm23 and p16 expression as biomarkers of high-risk human papillomavirus infection and cervical intraepithelial neoplasia 2+ lesions of the uterine cervix
AU - Benevolo, Maria
AU - Terrenato, Irene
AU - Mottolese, Marcella
AU - Marandino, Ferdinando
AU - Muti, Paola
AU - Carosi, Mariantonia
AU - Rollo, Francesca
AU - Ronchetti, Livia
AU - Mariani, Luciano
AU - Vocaturo, Giuseppe
AU - Vocaturo, Amina
PY - 2010/10
Y1 - 2010/10
N2 - Aims: To investigate the clinical role of nm23 expression in identifying both high-risk human papillomavirus (HR-HPV) and high-grade cervical lesions or carcinomas [cervical intraepithelial neoplasia 2+ (CIN2+)], and to compare it with p16 overexpression, as this latter biomarker has already been reported widely in HR-HPV infected cervical lesions.Methods and results: Immunohistochemical evaluation of nm23 and p16 in 143 cervical biopsy specimens including negative, low- and high-grade lesions and squamous carcinomas (SC). HR-HPV testing by Digene hybrid capture 2 (HC2) and polymerase chain reaction (PCR) on the cervico-vaginal samples of the same patients. In detecting CIN2+, p16 was significantly more sensitive and specific than nm23 (96.3% versus 81.8% and 66% versus 36.4%, respectively, both P <0.0001). Concerning HR-HPV detection by HC2, p16 showed a significantly higher specificity than nm23 (82% versus 47%, P + or HR-HPV infection.
AB - Aims: To investigate the clinical role of nm23 expression in identifying both high-risk human papillomavirus (HR-HPV) and high-grade cervical lesions or carcinomas [cervical intraepithelial neoplasia 2+ (CIN2+)], and to compare it with p16 overexpression, as this latter biomarker has already been reported widely in HR-HPV infected cervical lesions.Methods and results: Immunohistochemical evaluation of nm23 and p16 in 143 cervical biopsy specimens including negative, low- and high-grade lesions and squamous carcinomas (SC). HR-HPV testing by Digene hybrid capture 2 (HC2) and polymerase chain reaction (PCR) on the cervico-vaginal samples of the same patients. In detecting CIN2+, p16 was significantly more sensitive and specific than nm23 (96.3% versus 81.8% and 66% versus 36.4%, respectively, both P <0.0001). Concerning HR-HPV detection by HC2, p16 showed a significantly higher specificity than nm23 (82% versus 47%, P + or HR-HPV infection.
KW - Cervical intraepithelial neoplasia
KW - Diagnostic biomarkers
KW - HPV testing
KW - Human papillomavirus
KW - Nm23
KW - P16
KW - Uterine cervix
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U2 - 10.1111/j.1365-2559.2010.03674.x
DO - 10.1111/j.1365-2559.2010.03674.x
M3 - Article
C2 - 20955383
AN - SCOPUS:78650481294
VL - 57
SP - 580
EP - 586
JO - Histopathology
JF - Histopathology
SN - 0309-0167
IS - 4
ER -