Comparative genomics of closely related strains of Klebsiella pneumoniae reveals genes possibly involved in colistin resistance

Davide Sassera, Francesco Comandatore, Paolo Gaibani, Giuseppe D'Auria, Mara Mariconti, Maria Paola Landini, Vittorio Sambri, Piero Marone

Research output: Contribution to journalArticle

Abstract

Strains of colistin-resistant Klebsiella pneumoniae are emerging worldwide, due to the increased use of this molecule in antibiotic-resistant nosocomial infections. Comparative genomics was performed on three closely related K. pneumoniae strains isolated from three patients in a single hospital in Bologna, Italy. Two of these isolates are colistinresistant, while the third is sensitive to this antibiotic. The designed bioinformatic approach detected, among the three analyzed genomes, single nucleotide polymorphisms, insertions and deletions, specific patterns of gene presence and absence, in a total of 270 genes. These genes were analyzed by automatic and manual methods, to identify those potentially involved in colistin resistance, based on the data available in the literature and on the mechanism of action of colistin, the alteration of the outer membrane. Three of the identified genes (waaL, rfbA, vacJ), all presenting non-synonymous substitutions in the colistin resistant strains, resulted to be of special interest, due to the specific function of their protein products, involved in the biosynthesis of the outer bacterial membrane.

Original languageEnglish
Pages (from-to)887-890
Number of pages4
JournalAnnals of Microbiology
Volume64
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • Klebsiella pneumoniae . Colistin resistance . waaL . rfbA . vacJ

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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