Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: cytotoxicity-dominated nickel versus pleiotrope pollen reaction

Kilian Eyerich, Raik Böckelmann, Ansgar J. Pommer, Stefanie Foerster, Henning Hofmeister, Johannes Huss-Marp, Andrea Cavani, Heidrun Behrendt, Johannes Ring, Harald Gollnick, Bernd Bonnekoh, Claudia Traidl-Hoffmann

Research output: Contribution to journalArticlepeer-review

Abstract

Please cite this paper as: Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: cytotoxicity-dominated nickel versus pleiotrope pollen reaction. Experimental Dermatology 2010; 19: 511-517. Abstract: A subgroup of patients with atopic eczema develops acute eczematous reactions to type I allergy-inducing agents such as pollen that clinically resemble type IV allergies induced by haptens like metal ions. To clarify the underlying immunologic mechanisms, this study was designed to map the inflammatory in situ topoproteome of eczematous responses to grass/birch pollen and nickel by using atopy patch test (APT) and nickel patch test (NPT) as an appropriate clinical model, respectively. Biopsies from NPT (n = 6) and APT (n = 6) with positive reactions at 72 h were analysed by multiple epitope ligand cartography (MELC), which enabled to investigate coexpression of 49 different epitopes immunohistochemically in a single given tissue section. Colocalisation of IgE and FcεRI was investigated by confocal microscopy. Compared with APT responses, NPT reactions were dominated by cytotoxic TIA-1 + and CD8 + T cells. In contrast, the immune response in APT reactions appeared more pleiotrope - as detected by colocalisation analysis. Multiple combinatorial molecular phenotype (CMP) motifs containing naive, early maturation and memory T cell (CD45RA, CD7, CD44, CD45R0), and general activation markers (CLA, HLA-DR, CD13, CD29, CD58, CD71, CD138) were significantly higher expressed in APT when compared with NPT reactions. APT response was confirmed to be accompanied by IgE bound to FcεRI. In summary, our results demonstrate that the NPT reaction is clearly dominated by cytotoxic events, while the APT reaction to pollen grains is more heterogeneous and elicits a combined humoral and cellular immune reaction.

Original languageEnglish
Pages (from-to)511-517
Number of pages7
JournalExperimental Dermatology
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2010

Keywords

  • Atopic dermatitis/eczema
  • Atopy patch test
  • In situ topoproteome analysis
  • Multiple epitope ligand cartography
  • Nickel
  • Pollen

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

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