Comparative in vitro and in vivo β-oxidation of N-nitrosodiethanolamine in different animal species

Marina Bonfanti, Cinzia Magagnotti, Roberto Fanelli, Luisa Airoldi

Research output: Contribution to journalArticle

Abstract

The metabolism of N-nitrosodiethanolamine (NDELA) was studied to assess whether the formation of the β-oxidated metabolites N-(2-hydroxyethyl)N-(formylmethyl)nitrosamine (EFMN) and N-(2-hydroxyethyl)-N-(carboxy-methyl)nitrosamine (ECMN) is involved in the mechanism of tumor induction in various animal species with different susceptibility to NDELA carcinogenicity. In vitro studies using liver S9 fractions from rats, hamster, B6C3F1 and CD-1 mice and rabbits showed that all the animal species metabolize NDELA through the β-oxidation pathway, although to different extents. Urinary excretion of NDELA and its metabolite ECMN in rats, hamsters and mice after 5 mg·kg-1 NDELA i.p. confirmed these findings. The results suggest there is no correlation between carcinogenesis by NDELA and its β-oxidation. The possibility that ECMN formation might represent a detoxifying metabolic pathway for NDELA is discussed.

Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalChemico-Biological Interactions
Volume59
Issue numberC
DOIs
Publication statusPublished - 1986

Keywords

  • Animal species
  • BSTFA
  • ECMN
  • EFMN
  • gas chromatography-thermal energy analysis
  • GC-TEA
  • In vitro-in vivo metabolism
  • N,O-bis(trimethylsilyl)trifluoroacetamide
  • N-(2-hydroxyethyl)-N-(carboxymethyl)nitrosamine
  • N-(2-hydroxyethyl)-N-(formylmethyl)nitrosamine
  • N-nitrosodiethanolamine
  • N-nitrosomorpholine
  • NDELA
  • NMOR
  • TMS
  • trimethylsilyl derivative
  • β-Oxidation

ASJC Scopus subject areas

  • Toxicology

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