Comparative in vitro sensitivity of human cholangiocarcinoma and colon adenocarcinoma cells to anticancer agents

Raffaella Villa, Daniela Gornati, Nadia Zaffaroni, Silvia Veneroni, Paolo Bidoli, Aldo Severini

Research output: Contribution to journalArticle

Abstract

We comparatively investigated the sensitivity of a human cholangiocarcinoma cell line (SG231) and an adenocarcinoma cell line (WiDr) to mitoxantrone (MX), taxol (TX), mitomycin C (MMC), doxorubicin (DX), cisplatin (CDDP) and 5-fluorouracil (5FU) by the sulforhodamine B assay. The lower susceptibility of SG231 to 5FU, to CDDP, to DX and to MMC than WiDr was observed, whereas the sensitivity of the two cell lines to MX and TX was similar. We also investigated the ability of a chemical modulator lonidamine (LND), and hyperthermia to enhance the cytotoxic activity of the different drugs in the SG231 cell line. No potentiation of MX or CDDP activity was observed after a 2 hours treatment in hyperthermic conditions (42°C). Conversely, a slight potentiation of a 2 hours pretreatment with MX and DX was obtained by a 24 hours post treatment with LND.

Original languageEnglish
Pages (from-to)961-968
Number of pages8
JournalAnticancer Research
Volume17
Issue number2 A
Publication statusPublished - 1997

Keywords

  • Anticancer agents
  • Cytotoxicity
  • Human cholangiocarcinoma
  • Human colon adenocarcinoma
  • Modulators
  • Sulforhodamine assay

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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