Comparative in vivo study of alloy titanium implants with two different surfaces: biomechanical and SEM analysis

Francisley Ávila Souza, Thayane Silveira Mata Furtado, Ulisses Ribeiro Campos Dayube, Willian Moraes Melo, Renato Sussumu Nishioka, Pier Paolo Poli, Carlo Maiorana, Paulo Sérgio Perri de Carvalho

Research output: Contribution to journalArticle

Abstract

Objectives: The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). Materials and methods: Before surgery, topographic characterization was performed by SEM-EDX and by mean roughness measurements. Ten Albinus rabbits received randomly 20 Ti-6Al-4V implants on its right and left tibiae, with one implant placed in each tibia. After implant insertion, the implant stability quotient (ISQ) was measured by means of resonance frequency analysis (RFA). After 3 and 6 weeks, the ISQ was again measured, followed by torque removal measurements. Analysis of variance and Tukey tests were used to analyze the data. The surface of the implants removed was evaluated by SEM-EDX. Immunohistochemical analysis of osteopontin (OPN) and osteocalcin (OC) protein was performed in bone tissue. Results: The topographic characterization showed differences between the analyzed surfaces, and the mean roughness values of SBAS group were statistically higher than MS. Overall, higher statistically significant ISQ values were observed in the SBAS group compared to the MS group (p = 0.012). The intra-group comparison of ISQ values in the SBAS group showed statistically significant differences between 0 and 3 weeks (p = 0.032) and 0 and 6 weeks (p = 0.003). The torque removal measurements of group SBAS were statistically higher when compared with the torque removal measurements of group MS in the time intervals of 3 weeks (p = 0.002) and 6 weeks (p < 0.001). SEM-EDX of the implant surfaces removed in SBAS group showed greater bone tissue covering and mean values atomic in percentage of Ca, P, and O statistically superior (p < 0.05) than MS group. Immunohistochemical reactions showed intense OC immunolabeling at 6 weeks postoperative for SBAS group. Conclusions: The topographical modifications made in group SBAS allowed a better mechanical interlocking between the implant and bone tissue.

Original languageEnglish
Pages (from-to)4383-4397
JournalClinical Oral Investigations
Volume23
Issue number12
DOIs
Publication statusPublished - 2019

Fingerprint

Titanium
Torque
Bone and Bones
Osteocalcin
Tibia
Osteopontin
Analysis of Variance
Rabbits
Acids
Proteins

Keywords

  • Biomechanics
  • Dental implant
  • Osseointegration
  • Surface modification

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Souza, F. Á., Furtado, T. S. M., Dayube, U. R. C., Melo, W. M., Nishioka, R. S., Poli, P. P., ... de Carvalho, P. S. P. (2019). Comparative in vivo study of alloy titanium implants with two different surfaces: biomechanical and SEM analysis. Clinical Oral Investigations, 23(12), 4383-4397. https://doi.org/10.1007/s00784-019-02872-6

Comparative in vivo study of alloy titanium implants with two different surfaces : biomechanical and SEM analysis. / Souza, Francisley Ávila; Furtado, Thayane Silveira Mata; Dayube, Ulisses Ribeiro Campos; Melo, Willian Moraes; Nishioka, Renato Sussumu; Poli, Pier Paolo; Maiorana, Carlo; de Carvalho, Paulo Sérgio Perri.

In: Clinical Oral Investigations, Vol. 23, No. 12, 2019, p. 4383-4397.

Research output: Contribution to journalArticle

Souza, FÁ, Furtado, TSM, Dayube, URC, Melo, WM, Nishioka, RS, Poli, PP, Maiorana, C & de Carvalho, PSP 2019, 'Comparative in vivo study of alloy titanium implants with two different surfaces: biomechanical and SEM analysis', Clinical Oral Investigations, vol. 23, no. 12, pp. 4383-4397. https://doi.org/10.1007/s00784-019-02872-6
Souza, Francisley Ávila ; Furtado, Thayane Silveira Mata ; Dayube, Ulisses Ribeiro Campos ; Melo, Willian Moraes ; Nishioka, Renato Sussumu ; Poli, Pier Paolo ; Maiorana, Carlo ; de Carvalho, Paulo Sérgio Perri. / Comparative in vivo study of alloy titanium implants with two different surfaces : biomechanical and SEM analysis. In: Clinical Oral Investigations. 2019 ; Vol. 23, No. 12. pp. 4383-4397.
@article{c0c2f25d607f4ae9b6e5d0c54731ca31,
title = "Comparative in vivo study of alloy titanium implants with two different surfaces: biomechanical and SEM analysis",
abstract = "Objectives: The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). Materials and methods: Before surgery, topographic characterization was performed by SEM-EDX and by mean roughness measurements. Ten Albinus rabbits received randomly 20 Ti-6Al-4V implants on its right and left tibiae, with one implant placed in each tibia. After implant insertion, the implant stability quotient (ISQ) was measured by means of resonance frequency analysis (RFA). After 3 and 6 weeks, the ISQ was again measured, followed by torque removal measurements. Analysis of variance and Tukey tests were used to analyze the data. The surface of the implants removed was evaluated by SEM-EDX. Immunohistochemical analysis of osteopontin (OPN) and osteocalcin (OC) protein was performed in bone tissue. Results: The topographic characterization showed differences between the analyzed surfaces, and the mean roughness values of SBAS group were statistically higher than MS. Overall, higher statistically significant ISQ values were observed in the SBAS group compared to the MS group (p = 0.012). The intra-group comparison of ISQ values in the SBAS group showed statistically significant differences between 0 and 3 weeks (p = 0.032) and 0 and 6 weeks (p = 0.003). The torque removal measurements of group SBAS were statistically higher when compared with the torque removal measurements of group MS in the time intervals of 3 weeks (p = 0.002) and 6 weeks (p < 0.001). SEM-EDX of the implant surfaces removed in SBAS group showed greater bone tissue covering and mean values atomic in percentage of Ca, P, and O statistically superior (p < 0.05) than MS group. Immunohistochemical reactions showed intense OC immunolabeling at 6 weeks postoperative for SBAS group. Conclusions: The topographical modifications made in group SBAS allowed a better mechanical interlocking between the implant and bone tissue.",
keywords = "Biomechanics, Dental implant, Osseointegration, Surface modification",
author = "Souza, {Francisley {\'A}vila} and Furtado, {Thayane Silveira Mata} and Dayube, {Ulisses Ribeiro Campos} and Melo, {Willian Moraes} and Nishioka, {Renato Sussumu} and Poli, {Pier Paolo} and Carlo Maiorana and {de Carvalho}, {Paulo S{\'e}rgio Perri}",
year = "2019",
doi = "10.1007/s00784-019-02872-6",
language = "English",
volume = "23",
pages = "4383--4397",
journal = "Clinical Oral Investigations",
issn = "1432-6981",
publisher = "Springer Verlag",
number = "12",

}

TY - JOUR

T1 - Comparative in vivo study of alloy titanium implants with two different surfaces

T2 - biomechanical and SEM analysis

AU - Souza, Francisley Ávila

AU - Furtado, Thayane Silveira Mata

AU - Dayube, Ulisses Ribeiro Campos

AU - Melo, Willian Moraes

AU - Nishioka, Renato Sussumu

AU - Poli, Pier Paolo

AU - Maiorana, Carlo

AU - de Carvalho, Paulo Sérgio Perri

PY - 2019

Y1 - 2019

N2 - Objectives: The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). Materials and methods: Before surgery, topographic characterization was performed by SEM-EDX and by mean roughness measurements. Ten Albinus rabbits received randomly 20 Ti-6Al-4V implants on its right and left tibiae, with one implant placed in each tibia. After implant insertion, the implant stability quotient (ISQ) was measured by means of resonance frequency analysis (RFA). After 3 and 6 weeks, the ISQ was again measured, followed by torque removal measurements. Analysis of variance and Tukey tests were used to analyze the data. The surface of the implants removed was evaluated by SEM-EDX. Immunohistochemical analysis of osteopontin (OPN) and osteocalcin (OC) protein was performed in bone tissue. Results: The topographic characterization showed differences between the analyzed surfaces, and the mean roughness values of SBAS group were statistically higher than MS. Overall, higher statistically significant ISQ values were observed in the SBAS group compared to the MS group (p = 0.012). The intra-group comparison of ISQ values in the SBAS group showed statistically significant differences between 0 and 3 weeks (p = 0.032) and 0 and 6 weeks (p = 0.003). The torque removal measurements of group SBAS were statistically higher when compared with the torque removal measurements of group MS in the time intervals of 3 weeks (p = 0.002) and 6 weeks (p < 0.001). SEM-EDX of the implant surfaces removed in SBAS group showed greater bone tissue covering and mean values atomic in percentage of Ca, P, and O statistically superior (p < 0.05) than MS group. Immunohistochemical reactions showed intense OC immunolabeling at 6 weeks postoperative for SBAS group. Conclusions: The topographical modifications made in group SBAS allowed a better mechanical interlocking between the implant and bone tissue.

AB - Objectives: The purpose of this study was to evaluate the biomechanical behavior of the interface formed between bone and implants with machined surfaces (MS) and those modified by Al2O3 sandblasting and acid etching (SBAS). Materials and methods: Before surgery, topographic characterization was performed by SEM-EDX and by mean roughness measurements. Ten Albinus rabbits received randomly 20 Ti-6Al-4V implants on its right and left tibiae, with one implant placed in each tibia. After implant insertion, the implant stability quotient (ISQ) was measured by means of resonance frequency analysis (RFA). After 3 and 6 weeks, the ISQ was again measured, followed by torque removal measurements. Analysis of variance and Tukey tests were used to analyze the data. The surface of the implants removed was evaluated by SEM-EDX. Immunohistochemical analysis of osteopontin (OPN) and osteocalcin (OC) protein was performed in bone tissue. Results: The topographic characterization showed differences between the analyzed surfaces, and the mean roughness values of SBAS group were statistically higher than MS. Overall, higher statistically significant ISQ values were observed in the SBAS group compared to the MS group (p = 0.012). The intra-group comparison of ISQ values in the SBAS group showed statistically significant differences between 0 and 3 weeks (p = 0.032) and 0 and 6 weeks (p = 0.003). The torque removal measurements of group SBAS were statistically higher when compared with the torque removal measurements of group MS in the time intervals of 3 weeks (p = 0.002) and 6 weeks (p < 0.001). SEM-EDX of the implant surfaces removed in SBAS group showed greater bone tissue covering and mean values atomic in percentage of Ca, P, and O statistically superior (p < 0.05) than MS group. Immunohistochemical reactions showed intense OC immunolabeling at 6 weeks postoperative for SBAS group. Conclusions: The topographical modifications made in group SBAS allowed a better mechanical interlocking between the implant and bone tissue.

KW - Biomechanics

KW - Dental implant

KW - Osseointegration

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=85072022287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072022287&partnerID=8YFLogxK

U2 - 10.1007/s00784-019-02872-6

DO - 10.1007/s00784-019-02872-6

M3 - Article

AN - SCOPUS:85072022287

VL - 23

SP - 4383

EP - 4397

JO - Clinical Oral Investigations

JF - Clinical Oral Investigations

SN - 1432-6981

IS - 12

ER -