Comparative metabolomics profiling of isogenic KRAS wild type and mutant NSCLC cells in vitro and in vivo

Laura Brunelli, Elisa Caiola, Mirko Marabese, Massimo Broggini, Roberta Pastorelli

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Oncogenes induce metabolic reprogramming on cancer cells. Recently, G12C KRAS mutation in isogenic NSCLC cell line has been shown to be a key player in promoting metabolic rewiring mainly through the regulation of glutamine metabolism to fuel growth and proliferation. Even though cell lines possessing many of the genetic backgrounds of the primary cancer they derive from could be a valuable pre-clinical model, they do not have the additional complexity present in the whole tumor that impact metabolism. This preliminary study is aimed to explore how cancer cell metabolism in culture might recapitulate the metabolic alterations present in vivo. Our result highlighted that the gross metabolic changes observed in G12C KRAS mutant cells growing in culture were also maintained in the derived xenograft model, suggesting that a simple in vitro cell model can give important insights into the metabolic alterations induced by cancer. This is of relevance for guiding effective targeting of those metabolic traits that underlie tumor progression and anticancer treatment responses.

Original languageEnglish
Article number28398
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Jun 22 2016

Fingerprint

Metabolomics
Neoplasms
Cell Line
Glutamine
Oncogenes
Heterografts
In Vitro Techniques
Mutation
Growth

ASJC Scopus subject areas

  • General

Cite this

@article{8043a32f39e44121b2607b25a81e9141,
title = "Comparative metabolomics profiling of isogenic KRAS wild type and mutant NSCLC cells in vitro and in vivo",
abstract = "Oncogenes induce metabolic reprogramming on cancer cells. Recently, G12C KRAS mutation in isogenic NSCLC cell line has been shown to be a key player in promoting metabolic rewiring mainly through the regulation of glutamine metabolism to fuel growth and proliferation. Even though cell lines possessing many of the genetic backgrounds of the primary cancer they derive from could be a valuable pre-clinical model, they do not have the additional complexity present in the whole tumor that impact metabolism. This preliminary study is aimed to explore how cancer cell metabolism in culture might recapitulate the metabolic alterations present in vivo. Our result highlighted that the gross metabolic changes observed in G12C KRAS mutant cells growing in culture were also maintained in the derived xenograft model, suggesting that a simple in vitro cell model can give important insights into the metabolic alterations induced by cancer. This is of relevance for guiding effective targeting of those metabolic traits that underlie tumor progression and anticancer treatment responses.",
author = "Laura Brunelli and Elisa Caiola and Mirko Marabese and Massimo Broggini and Roberta Pastorelli",
year = "2016",
month = "6",
day = "22",
doi = "10.1038/srep28398",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Comparative metabolomics profiling of isogenic KRAS wild type and mutant NSCLC cells in vitro and in vivo

AU - Brunelli, Laura

AU - Caiola, Elisa

AU - Marabese, Mirko

AU - Broggini, Massimo

AU - Pastorelli, Roberta

PY - 2016/6/22

Y1 - 2016/6/22

N2 - Oncogenes induce metabolic reprogramming on cancer cells. Recently, G12C KRAS mutation in isogenic NSCLC cell line has been shown to be a key player in promoting metabolic rewiring mainly through the regulation of glutamine metabolism to fuel growth and proliferation. Even though cell lines possessing many of the genetic backgrounds of the primary cancer they derive from could be a valuable pre-clinical model, they do not have the additional complexity present in the whole tumor that impact metabolism. This preliminary study is aimed to explore how cancer cell metabolism in culture might recapitulate the metabolic alterations present in vivo. Our result highlighted that the gross metabolic changes observed in G12C KRAS mutant cells growing in culture were also maintained in the derived xenograft model, suggesting that a simple in vitro cell model can give important insights into the metabolic alterations induced by cancer. This is of relevance for guiding effective targeting of those metabolic traits that underlie tumor progression and anticancer treatment responses.

AB - Oncogenes induce metabolic reprogramming on cancer cells. Recently, G12C KRAS mutation in isogenic NSCLC cell line has been shown to be a key player in promoting metabolic rewiring mainly through the regulation of glutamine metabolism to fuel growth and proliferation. Even though cell lines possessing many of the genetic backgrounds of the primary cancer they derive from could be a valuable pre-clinical model, they do not have the additional complexity present in the whole tumor that impact metabolism. This preliminary study is aimed to explore how cancer cell metabolism in culture might recapitulate the metabolic alterations present in vivo. Our result highlighted that the gross metabolic changes observed in G12C KRAS mutant cells growing in culture were also maintained in the derived xenograft model, suggesting that a simple in vitro cell model can give important insights into the metabolic alterations induced by cancer. This is of relevance for guiding effective targeting of those metabolic traits that underlie tumor progression and anticancer treatment responses.

UR - http://www.scopus.com/inward/record.url?scp=84975828010&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975828010&partnerID=8YFLogxK

U2 - 10.1038/srep28398

DO - 10.1038/srep28398

M3 - Article

VL - 6

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 28398

ER -