Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice

Giselle Penton-Rol, Nielsen Lagumersindez-Denis, Luca Muzio, A. Bergami, Roberto Furlan, Julio R. Fernández-Massó, Marcelo Nazábal-Gálvez, Alexey Llópiz-Arzuaga, Tania Herrera-Rolo, T. Veliz Rodriguez, Nadia Polentarutti, Javier Marín-Prida, Ivette Raíces-Cruz, Carmen Valenzuela-Silva, Mauro Martins Teixeira, Eduardo Pentón-Arias

Research output: Contribution to journalArticle

Abstract

Multiple Sclerosis (MS) therapies approved so far are unable to effectively reverse the chronic phase of the disease or improve the remyelination process. Here our aim is to evaluate the effects of C-Phycocyanin (C-Pc), a biliprotein from Spirulina platensis with anti-oxidant, anti-inflammatory and cytoprotective properties, in a chronic model of experimental autoimmune encephalomyelitis (EAE) in mice. C-Pc (2, 4 or 8 mg/kg i.p.) or IFN-beta (2000 IU, s.c.) was administered daily once a day or every other day, respectively, starting at disease onset, which differ among EAE mice between 11 and 15 days postinduction. Histological and immunohistochemistry (anti-Mac-3, anti-CD3 and anti-APP) assessments were performed in spinal cord in the postinduction time. Global gene expression in the brain was analyzed with the Illumina Mouse WG-6_V2 BeadChip microarray and the expression of particular genes, assessed by qPCR using the Fast SYBR Green RT-PCR Master Mix. Oxidative stress parameters (malondialdehyde, peroxidation potential, CAT/SOD ratio and GSH) were determined spectrophoto-metrically. Results showed that C-Pc ameliorates the clinical deterioration of animals, an effect that expresses the reduction of the inflammatory infiltrates invading the spinal cord tissue, the axonal preservation and the down-regulation of IL-17 expression in brain tissue and serum. C-Pc and IFN-beta improved the redox status in mice subjected to EAE, while microarray analysis showed that both treatments shared a common subset of differentially expressed genes, although they also differentially modulated another subset of genes. Specifically, C-Pc mainly modulated the expression of genes related to remyelination, gliogenesis and axon-glia processes. Taken together, our results indicate that C-Pc has significant therapeutic effects against EAE, mediated by the dynamic regulation of multiple biological processes.

Original languageEnglish
Pages (from-to)153-167
Number of pages15
JournalJournal of NeuroImmune Pharmacology
Volume11
Issue number1
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Phycocyanin
Multiple Sclerosis
Autoimmune Experimental Encephalomyelitis
Gene Expression
Spinal Cord
Spirulina
Tissue Preservation
Biological Phenomena
Interleukin-17
Brain
Therapeutic Uses
Microarray Analysis
Malondialdehyde
Oxidants
Neuroglia
Genes
Oxidation-Reduction
Axons
Oxidative Stress
Chronic Disease

Keywords

  • C-Phycocyanin
  • EAE
  • IFN-beta
  • Multiple sclerosis
  • Neuroregeneration
  • Remyelination

ASJC Scopus subject areas

  • Pharmacology
  • Immunology and Allergy
  • Immunology
  • Neuroscience (miscellaneous)

Cite this

Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice. / Penton-Rol, Giselle; Lagumersindez-Denis, Nielsen; Muzio, Luca; Bergami, A.; Furlan, Roberto; Fernández-Massó, Julio R.; Nazábal-Gálvez, Marcelo; Llópiz-Arzuaga, Alexey; Herrera-Rolo, Tania; Veliz Rodriguez, T.; Polentarutti, Nadia; Marín-Prida, Javier; Raíces-Cruz, Ivette; Valenzuela-Silva, Carmen; Teixeira, Mauro Martins; Pentón-Arias, Eduardo.

In: Journal of NeuroImmune Pharmacology, Vol. 11, No. 1, 01.03.2016, p. 153-167.

Research output: Contribution to journalArticle

Penton-Rol, G, Lagumersindez-Denis, N, Muzio, L, Bergami, A, Furlan, R, Fernández-Massó, JR, Nazábal-Gálvez, M, Llópiz-Arzuaga, A, Herrera-Rolo, T, Veliz Rodriguez, T, Polentarutti, N, Marín-Prida, J, Raíces-Cruz, I, Valenzuela-Silva, C, Teixeira, MM & Pentón-Arias, E 2016, 'Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice', Journal of NeuroImmune Pharmacology, vol. 11, no. 1, pp. 153-167. https://doi.org/10.1007/s11481-015-9642-9
Penton-Rol, Giselle ; Lagumersindez-Denis, Nielsen ; Muzio, Luca ; Bergami, A. ; Furlan, Roberto ; Fernández-Massó, Julio R. ; Nazábal-Gálvez, Marcelo ; Llópiz-Arzuaga, Alexey ; Herrera-Rolo, Tania ; Veliz Rodriguez, T. ; Polentarutti, Nadia ; Marín-Prida, Javier ; Raíces-Cruz, Ivette ; Valenzuela-Silva, Carmen ; Teixeira, Mauro Martins ; Pentón-Arias, Eduardo. / Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice. In: Journal of NeuroImmune Pharmacology. 2016 ; Vol. 11, No. 1. pp. 153-167.
@article{ebde3b7a5995405f8a5bea61ee178dae,
title = "Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice",
abstract = "Multiple Sclerosis (MS) therapies approved so far are unable to effectively reverse the chronic phase of the disease or improve the remyelination process. Here our aim is to evaluate the effects of C-Phycocyanin (C-Pc), a biliprotein from Spirulina platensis with anti-oxidant, anti-inflammatory and cytoprotective properties, in a chronic model of experimental autoimmune encephalomyelitis (EAE) in mice. C-Pc (2, 4 or 8 mg/kg i.p.) or IFN-beta (2000 IU, s.c.) was administered daily once a day or every other day, respectively, starting at disease onset, which differ among EAE mice between 11 and 15 days postinduction. Histological and immunohistochemistry (anti-Mac-3, anti-CD3 and anti-APP) assessments were performed in spinal cord in the postinduction time. Global gene expression in the brain was analyzed with the Illumina Mouse WG-6_V2 BeadChip microarray and the expression of particular genes, assessed by qPCR using the Fast SYBR Green RT-PCR Master Mix. Oxidative stress parameters (malondialdehyde, peroxidation potential, CAT/SOD ratio and GSH) were determined spectrophoto-metrically. Results showed that C-Pc ameliorates the clinical deterioration of animals, an effect that expresses the reduction of the inflammatory infiltrates invading the spinal cord tissue, the axonal preservation and the down-regulation of IL-17 expression in brain tissue and serum. C-Pc and IFN-beta improved the redox status in mice subjected to EAE, while microarray analysis showed that both treatments shared a common subset of differentially expressed genes, although they also differentially modulated another subset of genes. Specifically, C-Pc mainly modulated the expression of genes related to remyelination, gliogenesis and axon-glia processes. Taken together, our results indicate that C-Pc has significant therapeutic effects against EAE, mediated by the dynamic regulation of multiple biological processes.",
keywords = "C-Phycocyanin, EAE, IFN-beta, Multiple sclerosis, Neuroregeneration, Remyelination",
author = "Giselle Penton-Rol and Nielsen Lagumersindez-Denis and Luca Muzio and A. Bergami and Roberto Furlan and Fern{\'a}ndez-Mass{\'o}, {Julio R.} and Marcelo Naz{\'a}bal-G{\'a}lvez and Alexey Ll{\'o}piz-Arzuaga and Tania Herrera-Rolo and {Veliz Rodriguez}, T. and Nadia Polentarutti and Javier Mar{\'i}n-Prida and Ivette Ra{\'i}ces-Cruz and Carmen Valenzuela-Silva and Teixeira, {Mauro Martins} and Eduardo Pent{\'o}n-Arias",
year = "2016",
month = "3",
day = "1",
doi = "10.1007/s11481-015-9642-9",
language = "English",
volume = "11",
pages = "153--167",
journal = "Journal of NeuroImmune Pharmacology",
issn = "1557-1890",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Comparative Neuroregenerative Effects of C-Phycocyanin and IFN-Beta in a Model of Multiple Sclerosis in Mice

AU - Penton-Rol, Giselle

AU - Lagumersindez-Denis, Nielsen

AU - Muzio, Luca

AU - Bergami, A.

AU - Furlan, Roberto

AU - Fernández-Massó, Julio R.

AU - Nazábal-Gálvez, Marcelo

AU - Llópiz-Arzuaga, Alexey

AU - Herrera-Rolo, Tania

AU - Veliz Rodriguez, T.

AU - Polentarutti, Nadia

AU - Marín-Prida, Javier

AU - Raíces-Cruz, Ivette

AU - Valenzuela-Silva, Carmen

AU - Teixeira, Mauro Martins

AU - Pentón-Arias, Eduardo

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Multiple Sclerosis (MS) therapies approved so far are unable to effectively reverse the chronic phase of the disease or improve the remyelination process. Here our aim is to evaluate the effects of C-Phycocyanin (C-Pc), a biliprotein from Spirulina platensis with anti-oxidant, anti-inflammatory and cytoprotective properties, in a chronic model of experimental autoimmune encephalomyelitis (EAE) in mice. C-Pc (2, 4 or 8 mg/kg i.p.) or IFN-beta (2000 IU, s.c.) was administered daily once a day or every other day, respectively, starting at disease onset, which differ among EAE mice between 11 and 15 days postinduction. Histological and immunohistochemistry (anti-Mac-3, anti-CD3 and anti-APP) assessments were performed in spinal cord in the postinduction time. Global gene expression in the brain was analyzed with the Illumina Mouse WG-6_V2 BeadChip microarray and the expression of particular genes, assessed by qPCR using the Fast SYBR Green RT-PCR Master Mix. Oxidative stress parameters (malondialdehyde, peroxidation potential, CAT/SOD ratio and GSH) were determined spectrophoto-metrically. Results showed that C-Pc ameliorates the clinical deterioration of animals, an effect that expresses the reduction of the inflammatory infiltrates invading the spinal cord tissue, the axonal preservation and the down-regulation of IL-17 expression in brain tissue and serum. C-Pc and IFN-beta improved the redox status in mice subjected to EAE, while microarray analysis showed that both treatments shared a common subset of differentially expressed genes, although they also differentially modulated another subset of genes. Specifically, C-Pc mainly modulated the expression of genes related to remyelination, gliogenesis and axon-glia processes. Taken together, our results indicate that C-Pc has significant therapeutic effects against EAE, mediated by the dynamic regulation of multiple biological processes.

AB - Multiple Sclerosis (MS) therapies approved so far are unable to effectively reverse the chronic phase of the disease or improve the remyelination process. Here our aim is to evaluate the effects of C-Phycocyanin (C-Pc), a biliprotein from Spirulina platensis with anti-oxidant, anti-inflammatory and cytoprotective properties, in a chronic model of experimental autoimmune encephalomyelitis (EAE) in mice. C-Pc (2, 4 or 8 mg/kg i.p.) or IFN-beta (2000 IU, s.c.) was administered daily once a day or every other day, respectively, starting at disease onset, which differ among EAE mice between 11 and 15 days postinduction. Histological and immunohistochemistry (anti-Mac-3, anti-CD3 and anti-APP) assessments were performed in spinal cord in the postinduction time. Global gene expression in the brain was analyzed with the Illumina Mouse WG-6_V2 BeadChip microarray and the expression of particular genes, assessed by qPCR using the Fast SYBR Green RT-PCR Master Mix. Oxidative stress parameters (malondialdehyde, peroxidation potential, CAT/SOD ratio and GSH) were determined spectrophoto-metrically. Results showed that C-Pc ameliorates the clinical deterioration of animals, an effect that expresses the reduction of the inflammatory infiltrates invading the spinal cord tissue, the axonal preservation and the down-regulation of IL-17 expression in brain tissue and serum. C-Pc and IFN-beta improved the redox status in mice subjected to EAE, while microarray analysis showed that both treatments shared a common subset of differentially expressed genes, although they also differentially modulated another subset of genes. Specifically, C-Pc mainly modulated the expression of genes related to remyelination, gliogenesis and axon-glia processes. Taken together, our results indicate that C-Pc has significant therapeutic effects against EAE, mediated by the dynamic regulation of multiple biological processes.

KW - C-Phycocyanin

KW - EAE

KW - IFN-beta

KW - Multiple sclerosis

KW - Neuroregeneration

KW - Remyelination

UR - http://www.scopus.com/inward/record.url?scp=84957431002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957431002&partnerID=8YFLogxK

U2 - 10.1007/s11481-015-9642-9

DO - 10.1007/s11481-015-9642-9

M3 - Article

VL - 11

SP - 153

EP - 167

JO - Journal of NeuroImmune Pharmacology

JF - Journal of NeuroImmune Pharmacology

SN - 1557-1890

IS - 1

ER -