Comparative proteome bioinformatics: Identification of a whole complement of putative protein tyrosine kinases in the model flowering plant Arabidopsis thaliana

Andrea Carpi, Giovanni Di Maira, Marco Vedovato, Valeria Rossi, Tiziana Naccari, Massimo Floriduz, Mario Terzi, Francesco Filippini

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Phosphorylation by protein tyrosine kinases is crucial to the control of growth and development of multicellular eukaryotes, including humans, and it also seems to play an important role in multicellular prokaryotes. A plant tyrosine-specific kinase has not been identified yet; hence, plants have been suggested to share with unicellular eukaryote yeast a tyrosine phosphorylation system where a limited number of stress proteins are tyrosyl-phosphorylated only by a few dual-specificity (serine/threonine and tyrosine) kinases. However, preliminary evidence obtained so far suggests that tyrosine phosphorylation in plants depends on the developmental conditions. Since sequencing of the genome of the model flowering plant Arabidopsis thaliana has been recently completed, we have performed a bioinformatic screening of the whole Arabidopsis proteome to identify a model complement of bona fide protein tyrosine kinases. In silico analyses suggest that <4% of Arabidopsis kinases are tyrosine-specific kinases, whose gene expression has been assessed by a preliminary polymerase chain reaction screening of an Arabidopsis cDNA library. Finally, immunological evidence confirms that the number of Arabidopsis proteins specifically phosphorylated on tyrosine residues is much higher than in yeast.

Original languageEnglish
Pages (from-to)1494-1503
Number of pages10
JournalProteomics
Volume2
Issue number11
DOIs
Publication statusPublished - Nov 1 2002

Fingerprint

Proteome
Bioinformatics
Computational Biology
Arabidopsis
Protein-Tyrosine Kinases
Phosphorylation
Tyrosine
Complement System Proteins
Yeast
Screening
Eukaryota
Phosphotransferases
Arabidopsis Proteins
Yeasts
Protein-Serine-Threonine Kinases
Polymerase chain reaction
Heat-Shock Proteins
Gene Library
Gene expression
Growth and Development

Keywords

  • Arabidopsis thaliana
  • Bioinformatics
  • Motif
  • PROSITE
  • Tyrosine kinase PRO 0257

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Comparative proteome bioinformatics : Identification of a whole complement of putative protein tyrosine kinases in the model flowering plant Arabidopsis thaliana. / Carpi, Andrea; Di Maira, Giovanni; Vedovato, Marco; Rossi, Valeria; Naccari, Tiziana; Floriduz, Massimo; Terzi, Mario; Filippini, Francesco.

In: Proteomics, Vol. 2, No. 11, 01.11.2002, p. 1494-1503.

Research output: Contribution to journalArticle

Carpi, Andrea ; Di Maira, Giovanni ; Vedovato, Marco ; Rossi, Valeria ; Naccari, Tiziana ; Floriduz, Massimo ; Terzi, Mario ; Filippini, Francesco. / Comparative proteome bioinformatics : Identification of a whole complement of putative protein tyrosine kinases in the model flowering plant Arabidopsis thaliana. In: Proteomics. 2002 ; Vol. 2, No. 11. pp. 1494-1503.
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