Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins

Paolo Carcoforo, Blendi Ura, Carlo Mischiati, Monica Squerzanti, Vincenzo Lanzara, Carlo Cervellati, Roberta Calza, Patrizia Polverino De Laureto, Erica Frare, Mattia Portinari, Giordana Feriotto, Serena Lanzara, Enzo Agostinelli, Carlo M. Bergamini

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Abstract

The aim of the present study was to analyze the protein composition of ductal breast carcinoma and the surrounding normal tissue in individual patients using comparative 2D proteomics and mass spectrometry to detect candidate disease biomarkers for diagnosis and prognosis. Samples of normal and cancerous tissue obtained form 28 patients were analyzed. Chaperonins and cytoskeletal proteins predominated among the 11 proteins for which major changes in abundance were detected. Of these 11 proteins with an altered expression, 2 had a decreased expression and 9 had an increased expression. In addition, the abundance of a few cytokeratins was also altered; however, they were not capable of serving as specific circulatory biomarkers. The proteins which we observed to exhibit an altered expression in infiltrating ductal breast carcinoma may be exploited as novel targets for therapeutic interventions or represent novel diagnostic/prognostic markers for the early detection of aggressive tumors, particularly those with multridrug-resistant phenotypes during the earlier stages of the disease.

Original languageEnglish
Pages (from-to)1700-1704
Number of pages5
JournalMolecular Medicine Reports
Volume7
Issue number5
DOIs
Publication statusPublished - May 2013

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Keywords

  • Breast cancer
  • Cancer biomarkers
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Cancer Research
  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Oncology

Cite this

Carcoforo, P., Ura, B., Mischiati, C., Squerzanti, M., Lanzara, V., Cervellati, C., Calza, R., De Laureto, P. P., Frare, E., Portinari, M., Feriotto, G., Lanzara, S., Agostinelli, E., & Bergamini, C. M. (2013). Comparative proteomic analysis of ductal breast carcinoma demonstrates an altered expression of chaperonins and cytoskeletal proteins. Molecular Medicine Reports, 7(5), 1700-1704. https://doi.org/10.3892/mmr.2013.1375