Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line

Flora Cimmino, Daniela Spano, Mario Capasso, Nicola Zambrano, Roberta Russo, Massimo Zollo, Achille Iolascon

Research output: Contribution to journalArticle

Abstract

Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer.

Original languageEnglish
Pages (from-to)2550-2564
Number of pages15
JournalJournal of Proteome Research
Volume6
Issue number7
DOIs
Publication statusPublished - Jul 2007

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Keywords

  • 2D-DIGE
  • All-trans-retinoic acid
  • Cell line
  • Human neuroblastoma
  • LAN-5
  • Neuroblastoma

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Cimmino, F., Spano, D., Capasso, M., Zambrano, N., Russo, R., Zollo, M., & Iolascon, A. (2007). Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line. Journal of Proteome Research, 6(7), 2550-2564. https://doi.org/10.1021/pr060701g