Comparative study of cytokine content in the plasma and wound exudate from children with severe burns

E. V. Mikhal'Chik, J. A. Piterskaya, L. Y. Budkevich, L. Yu Pen'Kov, A. Facchiano, C. De Luca, G. A. Ibragimova, L. G. Korkina

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Abstract

The content of 27 cytokines was measured in blood plasma from 19 children with severe uncomplicated burns (group 1) and complicated burns (septic toxemia, toxemia, and pneumonia; group 2). Before surgical treatment (day 4 (±2) after burn), significant differences were found in the concentrations of interleukin-1 receptor antagonist, interleukin-6, interleukin-8, interleukin-10, tumor necrosis factor-α, interferon-γ, MCP-1, and granulocyte colony-stimulating factor. Cytokine concentration in group 2 patients was much higher than in group 1 patients and healthy children. The concentrations of interleukin-6, interleukin-8, and MCP-1 in group 1 patients significantly surpassed the normal level. Cytokine concentration in the plasma and wound exudates and myeloperoxidase activity in wound exudates from 4 patients of group 2 were measured over 18 days after burn. The inflammatory response was characterized by an increase in the content of interleukin-1β, interleukin-8, MCP-1, tumor necrosis factor-α, MIP-1α, and granulocyte-macrophage colony-stimulating factor in the wound (as compared to that in the plasma). Activity of myeloperoxidase in all patients was shown to correlate with the amount of MIP-1α (r=0.47), tumor necrosis factor-α (r=0.47), and granulocyte-macrophage colony-stimulating factor (r=0.55, p

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalBulletin of Experimental Biology and Medicine
Volume148
Issue number5
DOIs
Publication statusPublished - Nov 2009

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Keywords

  • Cytokines
  • Myeloperoxidase
  • Severe burns
  • Wound exudate

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Mikhal'Chik, E. V., Piterskaya, J. A., Budkevich, L. Y., Pen'Kov, L. Y., Facchiano, A., De Luca, C., Ibragimova, G. A., & Korkina, L. G. (2009). Comparative study of cytokine content in the plasma and wound exudate from children with severe burns. Bulletin of Experimental Biology and Medicine, 148(5), 771-775. https://doi.org/10.1007/s10517-010-0813-7