The 7-nitro group of nitrazepam (1,3-dihydro-7-nitro-5-phenyl-2H-1,4-benzodiazepin-2-one) was reduced to the 7-amino derivative and then acetylated to the 7-acetylamino derivative during perfusion of the isolated rat liver. These compounds were present mainly in perfusion fluid, only small amounts were found in bile or liver tissue, homogenized at the end of the experiment. Other metabolites were not detected with the techniques used. Similar results were found with livers of guinea-pigs and rabbits. In contrast, the 7-amino derivative was the final product of nitrazepam metabolism in perfused mouse livers.
- Nitrazepam metabolism
- Perfused liver
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience