Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia

M. Vitale, P. Tazzari, F. Ricci, M. A. Mazza, G. Zauli, G. Martini, L. Caimi, F. A. Manzoli, R. Conte

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Heparin-induced thrombocytopenia (HIT) is a possible complication of heparin therapy that can evolve with life-threatening thromboembolism, for which early diagnosis is essential. However, the specific laboratory approach to the diagnosis of HIT is still controversial. Methods: Sera from 13 patients with HIT, from 15 patients with non-HIT thrombocytopenia, and from 10 normal subjects were used to compare nonfunctional and functional methods to detect anti-heparin: PF-4 antibodies and platelet activation. We used three enzyme-linked immunosorbent assays (ELISAs) and the particle gel immunoassay as nonfunctional tests, and platelet aggregometry, CD62p (p-selectin) phenotypical expression, and Annexin V binding as functional assays. Results: CD62p expression was positive in 85% of the cases and Annexin V was positive in 40% of the HIT cases examined. Aggregometry gave variable results that depend strongly on the donor. Conclusion: Functional tests for platelet activation are more reliable for HIT diagnosis than the nonfunctional tests. We conclude that the phenotypical expression of p-selectin detected by flow cytometry on activated platelets appears to be a good functional marker for the diagnosis of HIT and its possible thromboembolic complications.

Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalCytometry
Volume46
Issue number5
DOIs
Publication statusPublished - Oct 15 2001

Fingerprint

Thrombocytopenia
Heparin
Selectins
Annexin A5
Platelet Activation
Blood Platelets
Thromboembolism
Immunoassay
Early Diagnosis
Flow Cytometry
Gels
Enzyme-Linked Immunosorbent Assay
Tissue Donors
Antibodies
Serum

Keywords

  • Annexin V
  • CD62p
  • Flow cytometry
  • Heparin-induced thrombocytopenia
  • Platelet activation

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Vitale, M., Tazzari, P., Ricci, F., Mazza, M. A., Zauli, G., Martini, G., ... Conte, R. (2001). Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia. Cytometry, 46(5), 290-295. https://doi.org/10.1002/cyto.1170

Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia. / Vitale, M.; Tazzari, P.; Ricci, F.; Mazza, M. A.; Zauli, G.; Martini, G.; Caimi, L.; Manzoli, F. A.; Conte, R.

In: Cytometry, Vol. 46, No. 5, 15.10.2001, p. 290-295.

Research output: Contribution to journalArticle

Vitale, M, Tazzari, P, Ricci, F, Mazza, MA, Zauli, G, Martini, G, Caimi, L, Manzoli, FA & Conte, R 2001, 'Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia', Cytometry, vol. 46, no. 5, pp. 290-295. https://doi.org/10.1002/cyto.1170
Vitale, M. ; Tazzari, P. ; Ricci, F. ; Mazza, M. A. ; Zauli, G. ; Martini, G. ; Caimi, L. ; Manzoli, F. A. ; Conte, R. / Comparison between different laboratory tests for the detection and prevention of heparin-induced thrombocytopenia. In: Cytometry. 2001 ; Vol. 46, No. 5. pp. 290-295.
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