Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.

G. Filice; G. Carnevale; V. Meroni; P. Ottoboni; S. Caligaris; G. Carosi

Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.

G. Filice, G. Carnevale, V. Meroni, P. Ottoboni, S. Caligaris, G. Carosi

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article › peer-review

Abstract

The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.

Original language	English
Pages (from-to)	311-314
Number of pages	4
Journal	Bollettino dell'Istituto Sieroterapico Milanese
Volume	64
Issue number	4
Publication status	Published - 1985

ASJC Scopus subject areas

Immunology

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title = "Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.",

abstract = "The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.",

author = "G. Filice and G. Carnevale and V. Meroni and P. Ottoboni and S. Caligaris and G. Carosi",

year = "1985",

language = "English",

volume = "64",

pages = "311--314",

journal = "Bollettino dell'Istituto Sieroterapico Milanese",

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T1 - Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.

AU - Filice, G.

AU - Carnevale, G.

AU - Meroni, V.

AU - Ottoboni, P.

AU - Caligaris, S.

AU - Carosi, G.

PY - 1985

Y1 - 1985

N2 - The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.

AB - The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.

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JO - Bollettino dell'Istituto Sieroterapico Milanese

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