Abstract
The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.
| Original language | English |
|---|---|
| Pages (from-to) | 311-314 |
| Number of pages | 4 |
| Journal | Bollettino dell'Istituto Sieroterapico Milanese |
| Volume | 64 |
| Issue number | 4 |
| Publication status | Published - 1985 |
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ASJC Scopus subject areas
- Immunology
Cite this
Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis. / Filice, G.; Carnevale, G.; Meroni, V.; Ottoboni, P.; Caligaris, S.; Carosi, G.
In: Bollettino dell'Istituto Sieroterapico Milanese, Vol. 64, No. 4, 1985, p. 311-314.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison between IgM-IFA and DS-IgM-ELISA performed on IgM fractions and whole sera in acute acquired toxoplasmosis.
AU - Filice, G.
AU - Carnevale, G.
AU - Meroni, V.
AU - Ottoboni, P.
AU - Caligaris, S.
AU - Carosi, G.
PY - 1985
Y1 - 1985
N2 - The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.
AB - The present investigation has shown that antibodies titers obtained by IgM-IFA performed on pure IgM-fractions, obtained by gel-filtration method, correlate well with DS-IgM-ELISA. False negative IgM-IFA results that frequently occurs with sera having greater than or equal to 200 I.U./ml by IgG-IFA, were not observed with DS-IgM-ELISA: and the 98% of sera positive only after separation of IgM fraction with IgM-IFA, are also positive with DS-IgM-ELISA performed on whole serum.
UR - http://www.scopus.com/inward/record.url?scp=0022308362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022308362&partnerID=8YFLogxK
M3 - Article
VL - 64
SP - 311
EP - 314
JO - Bollettino dell'Istituto Sieroterapico Milanese
JF - Bollettino dell'Istituto Sieroterapico Milanese
SN - 0021-2547
IS - 4
ER -