Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas

E. Sabattini, J. Gerdes, F. Gherlinzoni, S. Poggi, L. Zucchini, G. Melilli, F. Grigioni, M. T. Del Vecchio, L. Leoncini, B. Falini, S. A. Pileri

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Abstract

The monoclonal antibody (MAb) Ki-67 detects a nuclear proliferation-associated antigen which corresponds to a non-histone protein with a molecular weight of 395 and 345 kD. Its prognostic relevance has been assessed in both lymphoid and non-lymphoid tumours. The MAb PC10 picks up the proliferating cell nuclear antigen (PCNA), which is a 36 kD nuclear protein associated with the cell cycle. Whereas Ki-67 works only in fresh material, PC10 detects a fixation-resistant epitope of PCNA. Preliminary data have revealed a linear relationship between Ki-67 and PC10 reactivity in normal lymphoid tissue and in non-Hodgkin's lymphomas (NHLs). We applied Ki-67 and PC10 to frozen and routine sections, respectively, from 25 examples of Hodgkin's disease (HD) (14 nodular sclerosis, 6 lymphocyte predominance, 5 mixed cellularity) and 100 NHLs (corresponding to the main varieties of the updated Kiel classification). The results obtained can be summarized as follows: (1) both MAbs gave rise to extremely variable results within the same category of NHLs; (2) most Hodgkin and Reed-Sternberg cells (50-98 per cent) were labelled by the reagents; (3) Ki-67 and PC10 stained a similar ratio of neoplastic cells in 65 and 76 per cent of NHL and HD cases, respectively; in the remaining instances, no correspondence was observedd, the PC10-positive elements usually outnumbering the Ki-67-positive ones significantly. These discrepancies, which might be due to low PCNA catabolism and/or PCNA expression by quiescent cells, underline the need for further kinetic and clinico-pathologic studies in order to define the specific relevance of PC10.

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalJournal of Pathology
Volume169
Issue number4
Publication statusPublished - 1993

Fingerprint

Non-Hodgkin's Lymphoma
Proliferating Cell Nuclear Antigen
Lymphoma
Monoclonal Antibodies
Hodgkin Disease
Reed-Sternberg Cells
Frozen Sections
Sclerosis
Lymphoid Tissue
Nuclear Proteins
Epitopes
Cell Cycle
Molecular Weight
Lymphocytes
Antigens
Neoplasms
Proteins

Keywords

  • Growth fraction
  • Hodgkin's disease
  • Immunohistochemistry
  • Ki-67 antigen
  • Non-Hodgkin's lymphoma
  • PCNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sabattini, E., Gerdes, J., Gherlinzoni, F., Poggi, S., Zucchini, L., Melilli, G., ... Pileri, S. A. (1993). Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas. Journal of Pathology, 169(4), 397-403.

Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas. / Sabattini, E.; Gerdes, J.; Gherlinzoni, F.; Poggi, S.; Zucchini, L.; Melilli, G.; Grigioni, F.; Del Vecchio, M. T.; Leoncini, L.; Falini, B.; Pileri, S. A.

In: Journal of Pathology, Vol. 169, No. 4, 1993, p. 397-403.

Research output: Contribution to journalArticle

Sabattini, E, Gerdes, J, Gherlinzoni, F, Poggi, S, Zucchini, L, Melilli, G, Grigioni, F, Del Vecchio, MT, Leoncini, L, Falini, B & Pileri, SA 1993, 'Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas', Journal of Pathology, vol. 169, no. 4, pp. 397-403.
Sabattini E, Gerdes J, Gherlinzoni F, Poggi S, Zucchini L, Melilli G et al. Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas. Journal of Pathology. 1993;169(4):397-403.
Sabattini, E. ; Gerdes, J. ; Gherlinzoni, F. ; Poggi, S. ; Zucchini, L. ; Melilli, G. ; Grigioni, F. ; Del Vecchio, M. T. ; Leoncini, L. ; Falini, B. ; Pileri, S. A. / Comparison between the monoclonal antibodies Ki-67 and PC10 in 125 malignant lymphomas. In: Journal of Pathology. 1993 ; Vol. 169, No. 4. pp. 397-403.
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abstract = "The monoclonal antibody (MAb) Ki-67 detects a nuclear proliferation-associated antigen which corresponds to a non-histone protein with a molecular weight of 395 and 345 kD. Its prognostic relevance has been assessed in both lymphoid and non-lymphoid tumours. The MAb PC10 picks up the proliferating cell nuclear antigen (PCNA), which is a 36 kD nuclear protein associated with the cell cycle. Whereas Ki-67 works only in fresh material, PC10 detects a fixation-resistant epitope of PCNA. Preliminary data have revealed a linear relationship between Ki-67 and PC10 reactivity in normal lymphoid tissue and in non-Hodgkin's lymphomas (NHLs). We applied Ki-67 and PC10 to frozen and routine sections, respectively, from 25 examples of Hodgkin's disease (HD) (14 nodular sclerosis, 6 lymphocyte predominance, 5 mixed cellularity) and 100 NHLs (corresponding to the main varieties of the updated Kiel classification). The results obtained can be summarized as follows: (1) both MAbs gave rise to extremely variable results within the same category of NHLs; (2) most Hodgkin and Reed-Sternberg cells (50-98 per cent) were labelled by the reagents; (3) Ki-67 and PC10 stained a similar ratio of neoplastic cells in 65 and 76 per cent of NHL and HD cases, respectively; in the remaining instances, no correspondence was observedd, the PC10-positive elements usually outnumbering the Ki-67-positive ones significantly. These discrepancies, which might be due to low PCNA catabolism and/or PCNA expression by quiescent cells, underline the need for further kinetic and clinico-pathologic studies in order to define the specific relevance of PC10.",
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