Comparison between the traditional and a rapid screening test for cryoimmunoglobulins detection

Federica Romitelli, Leopoldo Paolo Pucillo, Umberto Basile, Enrico Di Stasio

Research output: Contribution to journalArticle

Abstract

Objectives. A new rapid, automatic, and sensitive screening test useful to detect cryoglobulins in serum samples is proposed. Design and Methods. The increase of turbidity during the cryoglobulin aggregation was monitored spectrophotometrically in sera from 400 patients with clinical evidence of cryoglobulinemia related disorders and 100 controls. Results were correlated to those obtained by the traditional method. Results. Kinetics of the aggregation curves were described by their maximum turbidity increase, lag time, and slope. Despite a partial correspondence between the traditional and the rapid test, patients with symptomatic cryoglobulinemia showed turbidity values significantly higher than the determined cutoff. Moreover, a functional classification of cryoglobulins is proposed. Conclusions. Due to its high reproducibility, operator independence, low cost, and results obtained within 2 hours, the rapid test can be used as a "real time" monitoring of cryoglobulinemia related diseases and for the evaluation of plasmapheresis efficacy.

Original languageEnglish
Article number783063
JournalBioMed Research International
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Cryoglobulins
Cryoglobulinemia
Turbidity
Screening
Agglomeration
Plasmapheresis
Serum
Costs and Cost Analysis
Kinetics
Monitoring
Costs

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Comparison between the traditional and a rapid screening test for cryoimmunoglobulins detection. / Romitelli, Federica; Pucillo, Leopoldo Paolo; Basile, Umberto; Di Stasio, Enrico.

In: BioMed Research International, Vol. 2015, 783063, 2015.

Research output: Contribution to journalArticle

Romitelli, Federica ; Pucillo, Leopoldo Paolo ; Basile, Umberto ; Di Stasio, Enrico. / Comparison between the traditional and a rapid screening test for cryoimmunoglobulins detection. In: BioMed Research International. 2015 ; Vol. 2015.
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