Comparison between two methods in the determination of circulating chromogranin A in neuroendocrine tumors (NETs): Results of a prospective multicenter observational study

Antonette Leon, M. Torta, R. Dittadi, E. degli Uberti, M. R. Ambrosio, G. Delle Fave, F. De Braud, P. Tomassetti, M. Gion, L. Dogliotti

Research output: Contribution to journalArticlepeer-review

Abstract

Several methods for analyzing CgA using either monoclonal or polyclonal antibodies have been developed, which differ in their diagnostic performance. The present paper describes the results of a prospective multicenter study aimed at comparing the clinical value of the two most widely used commercially available CgA assay kits in patients affected by neuroendocrine tumors (NETs). Two hundred sixty-one patients from 40 different centers and 99 healthy subjects were evaluated. CgA levels were measured with two different methods, a two-step immunoradiometric assay (IRMA) and an enzyme-linked immunosorbent assay (ELISA). CgA was measured centrally by two reference laboratories, one of which used IRMA and the other ELISA, and it was measured by the participating institutions with the method routinely used by each of them. The major findings of the present study were: (i) the two assays for the determination of CgA present good diagnostic performance; (ii) both assays are robust and guarantee comparable results when applied in different settings (central vs local laboratory); (iii) the negative/positive cutoff points (87 ng/mL for IRMA and 21.3 U/L for ELISA) were established according to standardized criteria; (iv) the results obtained with the two assays in basal clinical samples of patients affected by NETs show an apparently satisfactory correlation (rs=0.843, p

Original languageEnglish
Pages (from-to)156-168
Number of pages13
JournalInternational Journal of Biological Markers
Volume20
Issue number3
Publication statusPublished - Jul 2005

Keywords

  • Chromogranin A
  • Cutoff point
  • Enzyme-linked immunosorbent assay (ELISA)
  • Immunoradiometic assay (IRMA)
  • Method comparison
  • Neuroendocrine tumors

ASJC Scopus subject areas

  • Immunology
  • Biochemistry

Fingerprint Dive into the research topics of 'Comparison between two methods in the determination of circulating chromogranin A in neuroendocrine tumors (NETs): Results of a prospective multicenter observational study'. Together they form a unique fingerprint.

Cite this