Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection

Paola Mantegani, Federica Piana, Laura Galli, Paolo Scarpellini, Luigi Codecasa, Adriano Lazzarin, Daniela Chillo, Claudio Fortis

Research output: Contribution to journalArticle

Abstract

Identification of individuals with a tuberculosis infection is a very important element for the control of tuberculosis. The currently used tuberculin skin test has poor sensitivity and specificity, Recently, an important advance in tuberculosis diagnosis occurred with the development of in vitro T cell-based IFN-γ release assays. The aim of this study was to compare a RD1-based in-house ELISPOT-IFN-γ assay with a commercial (T-SPOT.TB) assay for the diagnosis of tuberculosis infection. The results showed an almost complete concordance between the two assays, confirming that our restricted but highly selected pool of peptides is sufficient to detect tuberculosis infection.

Original languageEnglish
Pages (from-to)291-294
Number of pages4
JournalNew Microbiologica
Volume30
Issue number3
Publication statusPublished - Jul 2007

Fingerprint

Mycobacterium Infections
Mycobacterium tuberculosis
Tuberculosis
T-Lymphocytes
Infection
Enzyme-Linked Immunospot Assay
Tuberculin Test
Skin Tests
Sensitivity and Specificity
Peptides

Keywords

  • Bacillus Calmette-Guérin
  • ELISPOT
  • IFN
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Mantegani, P., Piana, F., Galli, L., Scarpellini, P., Codecasa, L., Lazzarin, A., ... Fortis, C. (2007). Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection. New Microbiologica, 30(3), 291-294.

Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection. / Mantegani, Paola; Piana, Federica; Galli, Laura; Scarpellini, Paolo; Codecasa, Luigi; Lazzarin, Adriano; Chillo, Daniela; Fortis, Claudio.

In: New Microbiologica, Vol. 30, No. 3, 07.2007, p. 291-294.

Research output: Contribution to journalArticle

Mantegani, P, Piana, F, Galli, L, Scarpellini, P, Codecasa, L, Lazzarin, A, Chillo, D & Fortis, C 2007, 'Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection', New Microbiologica, vol. 30, no. 3, pp. 291-294.
Mantegani, Paola ; Piana, Federica ; Galli, Laura ; Scarpellini, Paolo ; Codecasa, Luigi ; Lazzarin, Adriano ; Chillo, Daniela ; Fortis, Claudio. / Comparison of a commercial and an in-house T cell-based assay for the diagnosis of Mycobacterium tuberculosis infection. In: New Microbiologica. 2007 ; Vol. 30, No. 3. pp. 291-294.
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