Comparison of alternative mesenchymal stem cell sources for cell banking and musculoskeletal advanced therapies

Carola Cavallo, Carmela Cuomo, Sara Fantini, Francesca Ricci, Pier Luigi Tazzari, Enrico Lucarelli, Davide Donati, Andrea Facchini, Gina Lisignoli, Pier Maria Fornasari, Brunella Grigolo, Lorenzo Moroni

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

With the continuous discovery of new alternative sources containing mesenchymal stem cells (MSCs), regenerative medicine therapies may find tailored applications in the clinics. Although these cells have been demonstrated to express specific mesenchymal markers and are able to differentiate into mesenchymal lineages in ad hoc culture conditions, it is still critical to determine the yield and differentiation potential of these cells in comparative studies under the same standardized culture environment. Moreover, the opportunity to use MSCs from bone marrow (BM) of multiorgan donors for cell banking is of relevant importance. In the attempt to establish the relative potential of alternative MSCs sources, we analyzed and compared the yield and differentiation potential of human MSCs from adipose and BM tissues of cadaveric origins, and from fetal annexes (placenta and umbilical cord) after delivery using standardized isolation and culture protocols. BM contained a significantly higher amount of mononuclear cells (MNCs) compared to the other tissue sources. Nonetheless, a higher cell seeding density was needed for these cells to successfully isolate MSCs. The MNCs populations were highly heterogeneous and expressed variable MSCs markers with a large variation from donor to donor. After MSCs selection through tissue culture plastic adhesion, cells displayed a comparable proliferation capacity with distinct colony morphologies and were positive for a pool of typical MSCs markers. In vitro differentiation assays showed a higher osteogenic differentiation capacity of adipose tissue and BM MSCs, and a higher chondrogenic differentiation capacity of BM MSCs.

Original languageEnglish
Pages (from-to)1418-1430
Number of pages13
JournalJournal of Cellular Biochemistry
Volume112
Issue number5
DOIs
Publication statusPublished - May 2011

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Bone
Bone Marrow
Therapeutics
Tissue
Tissue culture
Regenerative Medicine
Umbilical Cord
Cell adhesion
Cell culture
Cell Adhesion
Placenta
Plastics
Adipose Tissue
Cell Differentiation
Assays
Cell Count
Bone and Bones

Keywords

  • adipose tissue
  • allogeneic therapies
  • alternative sources
  • bone
  • cartilage
  • mesenchymal stem cells
  • placenta
  • regenerative medicine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Comparison of alternative mesenchymal stem cell sources for cell banking and musculoskeletal advanced therapies. / Cavallo, Carola; Cuomo, Carmela; Fantini, Sara; Ricci, Francesca; Tazzari, Pier Luigi; Lucarelli, Enrico; Donati, Davide; Facchini, Andrea; Lisignoli, Gina; Fornasari, Pier Maria; Grigolo, Brunella; Moroni, Lorenzo.

In: Journal of Cellular Biochemistry, Vol. 112, No. 5, 05.2011, p. 1418-1430.

Research output: Contribution to journalArticle

Cavallo, Carola ; Cuomo, Carmela ; Fantini, Sara ; Ricci, Francesca ; Tazzari, Pier Luigi ; Lucarelli, Enrico ; Donati, Davide ; Facchini, Andrea ; Lisignoli, Gina ; Fornasari, Pier Maria ; Grigolo, Brunella ; Moroni, Lorenzo. / Comparison of alternative mesenchymal stem cell sources for cell banking and musculoskeletal advanced therapies. In: Journal of Cellular Biochemistry. 2011 ; Vol. 112, No. 5. pp. 1418-1430.
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