Comparison of an improved RAPD fingerprinting with different typing methods for discriminating clinical isolates of Staphylococcus spp.

Giuseppe Damiani, Silvana Telecco, Sergio Comincini, Massimo Sironi, Edoardo Carretto, Piero Marone

Research output: Contribution to journalArticle

Abstract

Different epidemiological markers were used to characterize 2 Staphylococcus epidermidis and 8 Staphylococcus aureus strains isolated from patients with severe infections. We compared random amplified polymorphic DNA (RAPD) fingerprints, biotypes, antibiotic assays, plasmid profiles and chromosomal DNA restriction endonuclease analysis (REA). Data analysis based on numerical taxonomy methods indicates that RAPD and REA give similar results allowing a good discrimination of the two species and of each isolate. The RAPD method is easier and faster than REA, but the reproducibility of RAPD fingerprints obtained in independent experiments can be problematic. We have found simple technical devices to improve the reproducibility of the RAPD procedure which is therefore a very useful tool in epidemiology for identification and characterization of Staphylococcus spp.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalEuropean Journal of Epidemiology
Volume12
Issue number2
DOIs
Publication statusPublished - 1996

Keywords

  • Epidemiology
  • RAPD
  • Staphylococcus spp.
  • Typing

ASJC Scopus subject areas

  • Epidemiology

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