Abstract
The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P
| Original language | English |
|---|---|
| Pages (from-to) | 419-426 |
| Number of pages | 8 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 26 |
| Issue number | 2 |
| Publication status | Published - Apr 2013 |
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Keywords
- Antibody-conjugated magnetic immunoassay
- Cyclosporine
- Immunosuppressants
- Liquid chromatography-tandem mass spectrometry
- Tacrolimus
- Therapeutic drug monitoring
ASJC Scopus subject areas
- Pharmacology
- Immunology
- Immunology and Allergy
Cite this
Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood. / Cangemi, Giuliana; Barco, S.; Bonifazio, P.; Maffia, A.; Agazzi, A.; Melioli, G.
In: International Journal of Immunopathology and Pharmacology, Vol. 26, No. 2, 04.2013, p. 419-426.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood
AU - Cangemi, Giuliana
AU - Barco, S.
AU - Bonifazio, P.
AU - Maffia, A.
AU - Agazzi, A.
AU - Melioli, G.
PY - 2013/4
Y1 - 2013/4
N2 - The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P
AB - The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P
KW - Antibody-conjugated magnetic immunoassay
KW - Cyclosporine
KW - Immunosuppressants
KW - Liquid chromatography-tandem mass spectrometry
KW - Tacrolimus
KW - Therapeutic drug monitoring
UR - http://www.scopus.com/inward/record.url?scp=84881233814&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84881233814&partnerID=8YFLogxK
M3 - Article
C2 - 23755756
AN - SCOPUS:84881233814
VL - 26
SP - 419
EP - 426
JO - International Journal of Immunopathology and Pharmacology
JF - International Journal of Immunopathology and Pharmacology
SN - 0394-6320
IS - 2
ER -