Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood

Giuliana Cangemi, S. Barco, P. Bonifazio, A. Maffia, A. Agazzi, G. Melioli

Research output: Contribution to journalArticle

Abstract

The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume26
Issue number2
Publication statusPublished - Apr 2013

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Tacrolimus
Tandem Mass Spectrometry
Immunoassay
Liquid Chromatography
Cyclosporine
Antibodies
Pediatrics
Clinical Pathology
Drug Monitoring
Regression Analysis

Keywords

  • Antibody-conjugated magnetic immunoassay
  • Cyclosporine
  • Immunosuppressants
  • Liquid chromatography-tandem mass spectrometry
  • Tacrolimus
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

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AU - Barco, S.

AU - Bonifazio, P.

AU - Maffia, A.

AU - Agazzi, A.

AU - Melioli, G.

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