Abstract
The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P
| Original language | English |
|---|---|
| Pages (from-to) | 419-426 |
| Number of pages | 8 |
| Journal | International Journal of Immunopathology and Pharmacology |
| Volume | 26 |
| Issue number | 2 |
| Publication status | Published - Apr 2013 |
Keywords
- Antibody-conjugated magnetic immunoassay
- Cyclosporine
- Immunosuppressants
- Liquid chromatography-tandem mass spectrometry
- Tacrolimus
- Therapeutic drug monitoring
ASJC Scopus subject areas
- Pharmacology
- Immunology
- Immunology and Allergy