Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood

Giuliana Cangemi; S. Barco; P. Bonifazio; A. Maffia; A. Agazzi; G. Melioli

Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood

Giuliana Cangemi, S. Barco, P. Bonifazio, A. Maffia, A. Agazzi, G. Melioli

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this work is to compare the results of a commercially available liquid chromatography tandem mass spectrometry (LC-MS/MS) method in a clinical pathology laboratory for routine Therapeutic Drug Monitoring (TDM) of cyclosporine (CsA) and tacrolimus (Tacr) in pediatric patients with those obtained with the current antibody-conjugated magnetic immunoassay (ACMIA). Whole blood levels of CsA (n= 135) and Tacr (n=100) were sequentially analyzed by using ACMIA and LC-MS/MS on pediatric transplanted patients. The differences were analyzed by using the Passing Bablok regression analysis and the Bland and Altman test. The LC-MS/MS method showed excellent reproducibility and lower limits of quantification compared to the ACMIA. A linear relationship between ACMIA and LC-MS/MS was obtained for both CsA (r= 0.9449; P

Original language	English
Pages (from-to)	419-426
Number of pages	8
Journal	International Journal of Immunopathology and Pharmacology
Volume	26
Issue number	2
Publication status	Published - Apr 2013

Keywords

Antibody-conjugated magnetic immunoassay
Cyclosporine
Immunosuppressants
Liquid chromatography-tandem mass spectrometry
Tacrolimus
Therapeutic drug monitoring

ASJC Scopus subject areas

Pharmacology
Immunology
Immunology and Allergy

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Comparison of antibody-conjugated magnetic immunoassay and liquid chromatography-tandem mass spectrometry for the measurement of cyclosporine and tacrolimus in whole blood. / Cangemi, Giuliana ; Barco, S.; Bonifazio, P.; Maffia, A.; Agazzi, A.; Melioli, G.

In: International Journal of Immunopathology and Pharmacology, Vol. 26, No. 2, 04.2013, p. 419-426.

Research output: Contribution to journal › Article › peer-review

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keywords = "Antibody-conjugated magnetic immunoassay, Cyclosporine, Immunosuppressants, Liquid chromatography-tandem mass spectrometry, Tacrolimus, Therapeutic drug monitoring",

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AU - Agazzi, A.

AU - Melioli, G.

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