Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia

R. Paroni, B. Comuzzi, C. Arcelloni, S. Brocco, S. De Kreutzenberg, A. Tiengo, A. Ciucci, P. Beck-Peccoz, S. Genovese

Research output: Contribution to journalArticle

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Abstract

Background: The diagnosis of 'factitious hypoglycemia' is essentially based on the disclosure of hypoglycemic agents in blood or urine. The aim of this study was to evaluate the performance of capillary electrophoresis (CE) as a quantitative method for determination of chlorpropamide, tolbutamide, glipizide, gliclazide, and glibenclamide in serum. Methods: Serum samples (1 mL), with internal standard added, were purified by solid-phase extraction on OASIS(TM) HLB cartridges (Waters), dried under reduced pressure, and reconstituted with 30-60 μL of acetonitrile:H2O. Analysis was carried out by micellar electrokinetic capillary chromatography in 5 mmol/L borate, 5 mmol/L phosphate, 75 mmol/L sodium cholate, pH 8.5, containing 25 mL/L methanol. Separation was accomplished in a 20 cm x 50 μm (i.d.) silica capillary at 25 °C and a constant voltage of +10 kV. Pharmacokinetics of gliclazide (80-mg tablet) in a diabetic patient were assayed by both HPLC and CE. Two hypoglycemic patients positive by HPLC analysis for unreported gliclazide and tolbutamide overdose were also screened by CE. Results: Separation of six drugs (including the internal standard) was accomplished in 5 man plus 5 man rinsing. The between-day CV of the ratio of the areas of the sulfonylurea drugs to internal standard was 2 ≥0.998) and recovery (≥80%) were good for all sulfonylurea drugs tested. Pharmacokinetic curves for gliclazide by CE and HPLC were superimposable. CE analysis confirmed the HPLC diagnosis of surreptitious abuse of gliclazide and tolbutamide. Conclusion: CE is a useful tool in the clinical chemistry and toxicology laboratory for drug monitoring and pharmacokinetic investigations. (C) 2000 American Association for Clinical Chemistry.

Original languageEnglish
Pages (from-to)1773-1780
Number of pages8
JournalClinical Chemistry
Volume46
Issue number11
Publication statusPublished - 2000

Fingerprint

Capillary electrophoresis
Gliclazide
Capillary Electrophoresis
Hypoglycemia
High Pressure Liquid Chromatography
Tolbutamide
Pharmacokinetics
Hypoglycemic Agents
Pharmaceutical Preparations
Micellar Electrokinetic Capillary Chromatography
Glipizide
Sodium Cholate
Chlorpropamide
Borates
Clinical Chemistry
Drug Monitoring
Glyburide
Solid Phase Extraction
Disclosure
Chromatography

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Paroni, R., Comuzzi, B., Arcelloni, C., Brocco, S., De Kreutzenberg, S., Tiengo, A., ... Genovese, S. (2000). Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia. Clinical Chemistry, 46(11), 1773-1780.

Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia. / Paroni, R.; Comuzzi, B.; Arcelloni, C.; Brocco, S.; De Kreutzenberg, S.; Tiengo, A.; Ciucci, A.; Beck-Peccoz, P.; Genovese, S.

In: Clinical Chemistry, Vol. 46, No. 11, 2000, p. 1773-1780.

Research output: Contribution to journalArticle

Paroni, R, Comuzzi, B, Arcelloni, C, Brocco, S, De Kreutzenberg, S, Tiengo, A, Ciucci, A, Beck-Peccoz, P & Genovese, S 2000, 'Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia', Clinical Chemistry, vol. 46, no. 11, pp. 1773-1780.
Paroni R, Comuzzi B, Arcelloni C, Brocco S, De Kreutzenberg S, Tiengo A et al. Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia. Clinical Chemistry. 2000;46(11):1773-1780.
Paroni, R. ; Comuzzi, B. ; Arcelloni, C. ; Brocco, S. ; De Kreutzenberg, S. ; Tiengo, A. ; Ciucci, A. ; Beck-Peccoz, P. ; Genovese, S. / Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia. In: Clinical Chemistry. 2000 ; Vol. 46, No. 11. pp. 1773-1780.
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T1 - Comparison of capillary electrophoresis with HPLC for diagnosis of factitious hypoglycemia

AU - Paroni, R.

AU - Comuzzi, B.

AU - Arcelloni, C.

AU - Brocco, S.

AU - De Kreutzenberg, S.

AU - Tiengo, A.

AU - Ciucci, A.

AU - Beck-Peccoz, P.

AU - Genovese, S.

PY - 2000

Y1 - 2000

N2 - Background: The diagnosis of 'factitious hypoglycemia' is essentially based on the disclosure of hypoglycemic agents in blood or urine. The aim of this study was to evaluate the performance of capillary electrophoresis (CE) as a quantitative method for determination of chlorpropamide, tolbutamide, glipizide, gliclazide, and glibenclamide in serum. Methods: Serum samples (1 mL), with internal standard added, were purified by solid-phase extraction on OASIS(TM) HLB cartridges (Waters), dried under reduced pressure, and reconstituted with 30-60 μL of acetonitrile:H2O. Analysis was carried out by micellar electrokinetic capillary chromatography in 5 mmol/L borate, 5 mmol/L phosphate, 75 mmol/L sodium cholate, pH 8.5, containing 25 mL/L methanol. Separation was accomplished in a 20 cm x 50 μm (i.d.) silica capillary at 25 °C and a constant voltage of +10 kV. Pharmacokinetics of gliclazide (80-mg tablet) in a diabetic patient were assayed by both HPLC and CE. Two hypoglycemic patients positive by HPLC analysis for unreported gliclazide and tolbutamide overdose were also screened by CE. Results: Separation of six drugs (including the internal standard) was accomplished in 5 man plus 5 man rinsing. The between-day CV of the ratio of the areas of the sulfonylurea drugs to internal standard was 2 ≥0.998) and recovery (≥80%) were good for all sulfonylurea drugs tested. Pharmacokinetic curves for gliclazide by CE and HPLC were superimposable. CE analysis confirmed the HPLC diagnosis of surreptitious abuse of gliclazide and tolbutamide. Conclusion: CE is a useful tool in the clinical chemistry and toxicology laboratory for drug monitoring and pharmacokinetic investigations. (C) 2000 American Association for Clinical Chemistry.

AB - Background: The diagnosis of 'factitious hypoglycemia' is essentially based on the disclosure of hypoglycemic agents in blood or urine. The aim of this study was to evaluate the performance of capillary electrophoresis (CE) as a quantitative method for determination of chlorpropamide, tolbutamide, glipizide, gliclazide, and glibenclamide in serum. Methods: Serum samples (1 mL), with internal standard added, were purified by solid-phase extraction on OASIS(TM) HLB cartridges (Waters), dried under reduced pressure, and reconstituted with 30-60 μL of acetonitrile:H2O. Analysis was carried out by micellar electrokinetic capillary chromatography in 5 mmol/L borate, 5 mmol/L phosphate, 75 mmol/L sodium cholate, pH 8.5, containing 25 mL/L methanol. Separation was accomplished in a 20 cm x 50 μm (i.d.) silica capillary at 25 °C and a constant voltage of +10 kV. Pharmacokinetics of gliclazide (80-mg tablet) in a diabetic patient were assayed by both HPLC and CE. Two hypoglycemic patients positive by HPLC analysis for unreported gliclazide and tolbutamide overdose were also screened by CE. Results: Separation of six drugs (including the internal standard) was accomplished in 5 man plus 5 man rinsing. The between-day CV of the ratio of the areas of the sulfonylurea drugs to internal standard was 2 ≥0.998) and recovery (≥80%) were good for all sulfonylurea drugs tested. Pharmacokinetic curves for gliclazide by CE and HPLC were superimposable. CE analysis confirmed the HPLC diagnosis of surreptitious abuse of gliclazide and tolbutamide. Conclusion: CE is a useful tool in the clinical chemistry and toxicology laboratory for drug monitoring and pharmacokinetic investigations. (C) 2000 American Association for Clinical Chemistry.

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