Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua

Stefania Boccia; Patrizia Laurenti; Emanuele Leoncini; Rosarita Amore; Sara Vincenti; Dario Arzani; Filippo Berloco; Federica Boninti; Stefania Bruno; Fabrizio Celani; Gianfranco Damiani; Paolo Di Giannantonio; Umberto Moscato; Brunella Posteraro; Romina Sezzatini; Alessia Vecchioni; Malgorzata Wachocka; Walter Ricciardi; Gianluigi Quaranta; Maria G iovanna Ficarra

Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua

Translated title of the contribution: Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy

Stefania Boccia, Patrizia Laurenti, Emanuele Leoncini, Rosarita Amore, Sara Vincenti, Dario Arzani, Filippo Berloco, Federica Boninti, Stefania Bruno, Fabrizio Celani, Gianfranco Damiani, Paolo Di Giannantonio, Umberto Moscato, Brunella Posteraro, Romina Sezzatini, Alessia Vecchioni, Malgorzata Wachocka, Walter Ricciardi, Gianluigi Quaranta, Maria G iovanna Ficarra

Istituto San Raffaele Pisana

Research output: Contribution to journal › Article › peer-review

Abstract

The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.

Translated title of the contribution	Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy
Original language	Italian
Pages (from-to)	569-576
Number of pages	8
Journal	Igiene e sanità pubblica
Volume	71
Issue number	6
Publication status	Published - Nov 1 2015

ASJC Scopus subject areas

Medicine(all)

Cite this

Boccia, S., Laurenti, P., Leoncini, E., Amore, R., Vincenti, S., Arzani, D., Berloco, F., Boninti, F., Bruno, S., Celani, F., Damiani, G., Di Giannantonio, P., Moscato, U., Posteraro, B., Sezzatini, R., Vecchioni, A., Wachocka, M., Ricciardi, W., Quaranta, G., & Ficarra, M. G. I. (2015). Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua. Igiene e sanità pubblica, 71(6), 569-576.

Boccia, S, Laurenti, P, Leoncini, E, Amore, R, Vincenti, S, Arzani, D, Berloco, F, Boninti, F, Bruno, S, Celani, F, Damiani, G, Di Giannantonio, P, Moscato, U, Posteraro, B, Sezzatini, R, Vecchioni, A, Wachocka, M, Ricciardi, W, Quaranta, G & Ficarra, MGI 2015, 'Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua', Igiene e sanità pubblica, vol. 71, no. 6, pp. 569-576.

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abstract = "The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting. ",

author = "Stefania Boccia and Patrizia Laurenti and Emanuele Leoncini and Rosarita Amore and Sara Vincenti and Dario Arzani and Filippo Berloco and Federica Boninti and Stefania Bruno and Fabrizio Celani and Gianfranco Damiani and {Di Giannantonio}, Paolo and Umberto Moscato and Brunella Posteraro and Romina Sezzatini and Alessia Vecchioni and Malgorzata Wachocka and Walter Ricciardi and Gianluigi Quaranta and Ficarra, {Maria G iovanna}",

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AU - Boccia, Stefania

AU - Laurenti, Patrizia

AU - Leoncini, Emanuele

AU - Amore, Rosarita

AU - Vincenti, Sara

AU - Arzani, Dario

AU - Berloco, Filippo

AU - Boninti, Federica

AU - Bruno, Stefania

AU - Celani, Fabrizio

AU - Damiani, Gianfranco

AU - Di Giannantonio, Paolo

AU - Moscato, Umberto

AU - Posteraro, Brunella

AU - Sezzatini, Romina

AU - Vecchioni, Alessia

AU - Wachocka, Malgorzata

AU - Ricciardi, Walter

AU - Quaranta, Gianluigi

AU - Ficarra, Maria G iovanna

PY - 2015/11/1

Y1 - 2015/11/1

N2 - The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.

AB - The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.

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JO - Igiene e Sanita Pubblica

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ER -

Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua

Abstract

ASJC Scopus subject areas

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