Confronto tra il metodo colturale standard e la real-time PCR per l'identificazione di Legionella pneumophila in campioni di acqua

Translated title of the contribution: Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy

Stefania Boccia, Patrizia Laurenti, Emanuele Leoncini, Rosarita Amore, Sara Vincenti, Dario Arzani, Filippo Berloco, Federica Boninti, Stefania Bruno, Fabrizio Celani, Gianfranco Damiani, Paolo Di Giannantonio, Umberto Moscato, Brunella Posteraro, Romina Sezzatini, Alessia Vecchioni, Malgorzata Wachocka, Walter Ricciardi, Gianluigi Quaranta, Maria G iovanna Ficarra

Research output: Contribution to journalArticlepeer-review

Abstract

The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.

Translated title of the contributionComparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy
Original languageItalian
Pages (from-to)569-576
Number of pages8
JournalIgiene e sanità pubblica
Volume71
Issue number6
Publication statusPublished - Nov 1 2015

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy'. Together they form a unique fingerprint.

Cite this