Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network

Claudia Mannu, Anna Gazzola, Elena Ciabatti, Fabio Fuligni, Marzia Cavalli, Irene Della Starza, Elisa Genuardi, Barbara Mantoan, Luigia Monitillo, Ilaria Del Giudice, Marco Ladetto, Gianluca Gaidano, Elena Sabattini, Stefano A. Pileri, Sara Galimberti, Pier Paolo Piccaluga, Italiana Linfomi Minimal Residual Disease Network Fondazione Italiana Linfomi Minimal Residual Disease Network

Research output: Contribution to journalArticle

Abstract

Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.

Original languageEnglish
Pages (from-to)400-410
Number of pages11
JournalLeukemia and Lymphoma
Volume57
Issue number2
DOIs
Publication statusPublished - Feb 1 2016

Keywords

  • DNA extraction
  • FIL MRD Network
  • PCR

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Mannu, C., Gazzola, A., Ciabatti, E., Fuligni, F., Cavalli, M., Della Starza, I., Genuardi, E., Mantoan, B., Monitillo, L., Del Giudice, I., Ladetto, M., Gaidano, G., Sabattini, E., Pileri, S. A., Galimberti, S., Piccaluga, P. P., & Fondazione Italiana Linfomi Minimal Residual Disease Network, I. L. M. R. D. N. (2016). Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network. Leukemia and Lymphoma, 57(2), 400-410. https://doi.org/10.3109/10428194.2014.914199