TY - JOUR
T1 - Comparison of different DNA extraction methods from peripheral blood cells
T2 - Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network
AU - Mannu, Claudia
AU - Gazzola, Anna
AU - Ciabatti, Elena
AU - Fuligni, Fabio
AU - Cavalli, Marzia
AU - Della Starza, Irene
AU - Genuardi, Elisa
AU - Mantoan, Barbara
AU - Monitillo, Luigia
AU - Del Giudice, Ilaria
AU - Ladetto, Marco
AU - Gaidano, Gianluca
AU - Sabattini, Elena
AU - Pileri, Stefano A.
AU - Galimberti, Sara
AU - Piccaluga, Pier Paolo
AU - Fondazione Italiana Linfomi Minimal Residual Disease Network, Italiana Linfomi Minimal Residual Disease Network
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.
AB - Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.
KW - DNA extraction
KW - FIL MRD Network
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=84954489266&partnerID=8YFLogxK
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U2 - 10.3109/10428194.2014.914199
DO - 10.3109/10428194.2014.914199
M3 - Article
AN - SCOPUS:84954489266
VL - 57
SP - 400
EP - 410
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
SN - 1042-8194
IS - 2
ER -