Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network

Claudia Mannu; Anna Gazzola; Elena Ciabatti; Fabio Fuligni; Marzia Cavalli; Irene Della Starza; Elisa Genuardi; Barbara Mantoan; Luigia Monitillo; Ilaria Del Giudice; Marco Ladetto; Gianluca Gaidano; Elena Sabattini; Stefano A. Pileri; Sara Galimberti; Pier Paolo Piccaluga; Italiana Linfomi Minimal Residual Disease Network Fondazione Italiana Linfomi Minimal Residual Disease Network

doi:10.3109/10428194.2014.914199

Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network

Claudia Mannu, Anna Gazzola, Elena Ciabatti, Fabio Fuligni, Marzia Cavalli, Irene Della Starza, Elisa Genuardi, Barbara Mantoan, Luigia Monitillo, Ilaria Del Giudice, Marco Ladetto, Gianluca Gaidano, Elena Sabattini, Stefano A. Pileri, Sara Galimberti, Pier Paolo Piccaluga, Italiana Linfomi Minimal Residual Disease Network Fondazione Italiana Linfomi Minimal Residual Disease Network

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article

Abstract

Genomic DNA extraction is a primary component of genomic research and diagnostic routine analysis. Recently, the importance of this process has been highlighted by the necessity to standardize the diagnostic procedure. In this regard, the Minimal Residual Disease (MRD) Network of the Fondazione Italiana Linfomi (FIL MRD Network) has performed a comparative study of four different commercially available kits for DNA extraction, applying them on a panel of cellular pellets, with the aim of defining possible technical recommendations in order to harmonize and standardize diagnostic procedures in the clinical setting. Overall, all four kits usually allowed the recovery of a significant quantity of high-quality DNA (in most conditions), although specific indications could be addressed for cellular pellets of different sizes.

Original language	English
Pages (from-to)	400-410
Number of pages	11
Journal	Leukemia and Lymphoma
Volume	57
Issue number	2
DOIs	https://doi.org/10.3109/10428194.2014.914199
Publication status	Published - Feb 1 2016

Keywords

DNA extraction
FIL MRD Network
PCR

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.3109/10428194.2014.914199

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Mannu, C., Gazzola, A., Ciabatti, E., Fuligni, F., Cavalli, M., Della Starza, I., Genuardi, E., Mantoan, B., Monitillo, L., Del Giudice, I., Ladetto, M., Gaidano, G., Sabattini, E., Pileri, S. A., Galimberti, S., Piccaluga, P. P., & Fondazione Italiana Linfomi Minimal Residual Disease Network, I. L. M. R. D. N. (2016). Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network. Leukemia and Lymphoma, 57(2), 400-410. https://doi.org/10.3109/10428194.2014.914199

Comparison of different DNA extraction methods from peripheral blood cells : Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network. / Mannu, Claudia; Gazzola, Anna; Ciabatti, Elena; Fuligni, Fabio; Cavalli, Marzia; Della Starza, Irene; Genuardi, Elisa; Mantoan, Barbara; Monitillo, Luigia; Del Giudice, Ilaria; Ladetto, Marco; Gaidano, Gianluca; Sabattini, Elena; Pileri, Stefano A.; Galimberti, Sara; Piccaluga, Pier Paolo; Fondazione Italiana Linfomi Minimal Residual Disease Network, Italiana Linfomi Minimal Residual Disease Network.

In: Leukemia and Lymphoma, Vol. 57, No. 2, 01.02.2016, p. 400-410.

Research output: Contribution to journal › Article

Mannu, C, Gazzola, A, Ciabatti, E, Fuligni, F, Cavalli, M, Della Starza, I, Genuardi, E, Mantoan, B, Monitillo, L, Del Giudice, I, Ladetto, M, Gaidano, G, Sabattini, E, Pileri, SA, Galimberti, S, Piccaluga, PP & Fondazione Italiana Linfomi Minimal Residual Disease Network, ILMRDN 2016, 'Comparison of different DNA extraction methods from peripheral blood cells: Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network', Leukemia and Lymphoma, vol. 57, no. 2, pp. 400-410. https://doi.org/10.3109/10428194.2014.914199

Mannu, Claudia ; Gazzola, Anna ; Ciabatti, Elena ; Fuligni, Fabio ; Cavalli, Marzia ; Della Starza, Irene ; Genuardi, Elisa ; Mantoan, Barbara ; Monitillo, Luigia ; Del Giudice, Ilaria ; Ladetto, Marco ; Gaidano, Gianluca ; Sabattini, Elena ; Pileri, Stefano A. ; Galimberti, Sara ; Piccaluga, Pier Paolo ; Fondazione Italiana Linfomi Minimal Residual Disease Network, Italiana Linfomi Minimal Residual Disease Network. / Comparison of different DNA extraction methods from peripheral blood cells : Advice from the Fondazione Italiana Linfomi Minimal Residual Disease Network. In: Leukemia and Lymphoma. 2016 ; Vol. 57, No. 2. pp. 400-410.

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