Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines

L. Curatolo, B. Azzarone, C. Fally Crepin, C. Fally-Crépin, L. Morasca, A. Macieira-Coelho

Research output: Contribution to journalArticle

Abstract

The expression of transformation parameters (inhibition of cell division during cell crowding, anchorage dependence, loss of fibrin clot retractile activity and secretion of plasminogen activator) was studied in a heterospecific cellular hybrid, made between established L (TK-) cells and the normal human MRC-5 cells. The hybrid nature of the cross was confirmed by the ability to incorporate [3H]-thymidine, by growth in selective HAT medium, by the identification of human chromosomes and by the expression on the surface of 100% of hybrid cells of a human glycoprotein, which is recognized by the 4F2 monoclonal antibody. The hybrid cultures showed cell cycle inhibition which became less stringent with increasing population doublings and the loss of human chromosomes. Fibrin clot retraction and anchorage dependence were absent in spite of the presence of many human chromosomes. The two properties were present or lost simultaneously in the normal parent cells and in the transformed parent or hybrid cells respectively. The human type of plasminogen activator was secreted even with very little human genetic material left, and a complete dissociation between fibrin clot retraction and production of plasminogen activator was observed. The data strengthen the hypothesis that transformation is a multistep process that involves complex genetic control and where cells progressively express different phenotypes and escape growth control.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalInternational Journal of Cancer
Volume31
Issue number2
DOIs
Publication statusPublished - 1983

Fingerprint

Clot Retraction
Fibrin
Plasminogen Activators
Human Chromosomes
Cell Line
Hybrid Cells
Crowding
Aptitude
Medical Genetics
Growth
Cell Division
Thymidine
Glycoproteins
Cell Cycle
Monoclonal Antibodies
Phenotype
Population
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines. / Curatolo, L.; Azzarone, B.; Fally Crepin, C.; Fally-Crépin, C.; Morasca, L.; Macieira-Coelho, A.

In: International Journal of Cancer, Vol. 31, No. 2, 1983, p. 249-255.

Research output: Contribution to journalArticle

Curatolo, L, Azzarone, B, Fally Crepin, C, Fally-Crépin, C, Morasca, L & Macieira-Coelho, A 1983, 'Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines', International Journal of Cancer, vol. 31, no. 2, pp. 249-255. https://doi.org/10.1002/ijc.2910310219
Curatolo, L. ; Azzarone, B. ; Fally Crepin, C. ; Fally-Crépin, C. ; Morasca, L. ; Macieira-Coelho, A. / Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines. In: International Journal of Cancer. 1983 ; Vol. 31, No. 2. pp. 249-255.
@article{2a4b76a91c4e4f07afb65f72fcb6f9d9,
title = "Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines",
abstract = "The expression of transformation parameters (inhibition of cell division during cell crowding, anchorage dependence, loss of fibrin clot retractile activity and secretion of plasminogen activator) was studied in a heterospecific cellular hybrid, made between established L (TK-) cells and the normal human MRC-5 cells. The hybrid nature of the cross was confirmed by the ability to incorporate [3H]-thymidine, by growth in selective HAT medium, by the identification of human chromosomes and by the expression on the surface of 100{\%} of hybrid cells of a human glycoprotein, which is recognized by the 4F2 monoclonal antibody. The hybrid cultures showed cell cycle inhibition which became less stringent with increasing population doublings and the loss of human chromosomes. Fibrin clot retraction and anchorage dependence were absent in spite of the presence of many human chromosomes. The two properties were present or lost simultaneously in the normal parent cells and in the transformed parent or hybrid cells respectively. The human type of plasminogen activator was secreted even with very little human genetic material left, and a complete dissociation between fibrin clot retraction and production of plasminogen activator was observed. The data strengthen the hypothesis that transformation is a multistep process that involves complex genetic control and where cells progressively express different phenotypes and escape growth control.",
author = "L. Curatolo and B. Azzarone and {Fally Crepin}, C. and C. Fally-Cr{\'e}pin and L. Morasca and A. Macieira-Coelho",
year = "1983",
doi = "10.1002/ijc.2910310219",
language = "English",
volume = "31",
pages = "249--255",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Comparison of fibrin clot retraction with other transformation parameters after hybridization of normal and established cell lines

AU - Curatolo, L.

AU - Azzarone, B.

AU - Fally Crepin, C.

AU - Fally-Crépin, C.

AU - Morasca, L.

AU - Macieira-Coelho, A.

PY - 1983

Y1 - 1983

N2 - The expression of transformation parameters (inhibition of cell division during cell crowding, anchorage dependence, loss of fibrin clot retractile activity and secretion of plasminogen activator) was studied in a heterospecific cellular hybrid, made between established L (TK-) cells and the normal human MRC-5 cells. The hybrid nature of the cross was confirmed by the ability to incorporate [3H]-thymidine, by growth in selective HAT medium, by the identification of human chromosomes and by the expression on the surface of 100% of hybrid cells of a human glycoprotein, which is recognized by the 4F2 monoclonal antibody. The hybrid cultures showed cell cycle inhibition which became less stringent with increasing population doublings and the loss of human chromosomes. Fibrin clot retraction and anchorage dependence were absent in spite of the presence of many human chromosomes. The two properties were present or lost simultaneously in the normal parent cells and in the transformed parent or hybrid cells respectively. The human type of plasminogen activator was secreted even with very little human genetic material left, and a complete dissociation between fibrin clot retraction and production of plasminogen activator was observed. The data strengthen the hypothesis that transformation is a multistep process that involves complex genetic control and where cells progressively express different phenotypes and escape growth control.

AB - The expression of transformation parameters (inhibition of cell division during cell crowding, anchorage dependence, loss of fibrin clot retractile activity and secretion of plasminogen activator) was studied in a heterospecific cellular hybrid, made between established L (TK-) cells and the normal human MRC-5 cells. The hybrid nature of the cross was confirmed by the ability to incorporate [3H]-thymidine, by growth in selective HAT medium, by the identification of human chromosomes and by the expression on the surface of 100% of hybrid cells of a human glycoprotein, which is recognized by the 4F2 monoclonal antibody. The hybrid cultures showed cell cycle inhibition which became less stringent with increasing population doublings and the loss of human chromosomes. Fibrin clot retraction and anchorage dependence were absent in spite of the presence of many human chromosomes. The two properties were present or lost simultaneously in the normal parent cells and in the transformed parent or hybrid cells respectively. The human type of plasminogen activator was secreted even with very little human genetic material left, and a complete dissociation between fibrin clot retraction and production of plasminogen activator was observed. The data strengthen the hypothesis that transformation is a multistep process that involves complex genetic control and where cells progressively express different phenotypes and escape growth control.

UR - http://www.scopus.com/inward/record.url?scp=0020657677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020657677&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910310219

DO - 10.1002/ijc.2910310219

M3 - Article

C2 - 6681807

AN - SCOPUS:0020657677

VL - 31

SP - 249

EP - 255

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 2

ER -