Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation

G. B. Fogazzi, M. Bajetta, G. Banfi, M. Mihatsch

Research output: Contribution to journalArticle

Abstract

Two types of tissue preparation for immunofluorescent staining were compared with sections of the same kidney. In one, formalin-fixed paraffin-embedded kidney sections were incubated with pronase (IF-PRON). The optimal pronase exposure for bright, specific IF and mild background IF, suitable for the whole set of antisera was determined (pronase 0.75 g/l of Tris buffer for 60 min) and this was used for the subsequent steps of the study. Snap-frozen sections were also stained (IF-FROZ). Positive and negative cases, IF intensity, distribution and location in IgA nephropathy, membranous nephropathy, proliferative lupus nephritis were compared by the 2 methods. The main antigens for each disease were adequately revealed by IF-PRON, so that a correct diagnosis was possible in all cases. IF-PRON was also applied to sections before and after prolonged storage of blocks in the files, to see whether or not retrospective analysis is possible. Only minor differences were found between the 2 series of sections. Finally, the exposure of the sections to the continuous fluorescent light showed that IF fading was less in fixed sections. We conclude that IF-PRON is a reliable method for renal pathology with some advantages over the IF-FROZ.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalPathology Research and Practice
Volume185
Issue number2
Publication statusPublished - 1989

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Pronase
Formaldehyde
Freezing
Kidney
Membranous Glomerulonephritis
Tromethamine
Lupus Nephritis
Frozen Sections
Paraffin
Immunoglobulin A
Immune Sera
Pathology
Staining and Labeling
Light
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fogazzi, G. B., Bajetta, M., Banfi, G., & Mihatsch, M. (1989). Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation. Pathology Research and Practice, 185(2), 225-230.

Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation. / Fogazzi, G. B.; Bajetta, M.; Banfi, G.; Mihatsch, M.

In: Pathology Research and Practice, Vol. 185, No. 2, 1989, p. 225-230.

Research output: Contribution to journalArticle

Fogazzi, GB, Bajetta, M, Banfi, G & Mihatsch, M 1989, 'Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation', Pathology Research and Practice, vol. 185, no. 2, pp. 225-230.
Fogazzi, G. B. ; Bajetta, M. ; Banfi, G. ; Mihatsch, M. / Comparison of immunofluorescent findings in kidney after snap-freezing and formalin fixation. In: Pathology Research and Practice. 1989 ; Vol. 185, No. 2. pp. 225-230.
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